Novel female-specific trans-spliced and alternative splice forms of dsx in the silkworm Bombyx mori

被引：16

作者：

Duan, Jianping ^{[1
]}

Xu, Hanfu ^{[1
]}

Wang, Feng ^{[1
]}

Ma, Sanyuan ^{[1
]}

Zha, Xingfu ^{[1
]}

Guo, Huizhen ^{[1
]}

Zhao, Ping ^{[1
]}

Xia, Qingyou ^{[1
]}

机构：

[1] Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 431卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Doublesex; Sexual development; Trans-splicing; Bombyx mori; SEX-DETERMINATION; DOUBLESEX GENE; DROSOPHILA-MELANOGASTER; DOMESTICATED SILKWORM; DIFFERENTIATION; EXPRESSION; DMRT1; MOUSE; IDENTIFICATION; CHROMOSOME;

D O I：

10.1016/j.bbrc.2012.12.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Bombyx mori doublesex gene (Bmdsx) plays an important role in somatic sexual development. Its premRNA splices in a sex-specific manner to generate two female-specific and one male-specific splice forms. The present study investigated six novel dsx variants generated by trans-splicing between female dsx transcripts and two additional novel genes, dsrl and dsr2. Expression analysis indicated that Bmdsx-dsr1 represented splicing noise, whereas dsr2, which trans-spliced with dsx to generate five variants, regulated the expression of the female-specific B. mori dsx transcript Bmdsx(F)s. We unexpectedly found a novel exon 2n insertion during Bmdsx transcription, which did not influence the validity of the novel protein, BmDSX(F3) Ectopic expression of BmDSX(F3) repressed the pheromone-binding protein gene and the testis-specific gene A2 in males, and activated of the storage protein 1 gene. Our findings suggest that trans-splicing is a novel regulatory function of Bmdsx, which participates in female sexual development by regulating the expression of three BmDSX(F) proteins. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：630 / 635

页数：6

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