Extraction, purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits

被引：16

作者：

Wang, Hongjun ^{[1
]}

Jiang, Hong ^{[1
]}

Wang, Shude ^{[1
]}

Li, Xinguo ^{[2
]}

Yao, Dan ^{[1
]}

Dong, Jingjing ^{[3
]}

Zhou, Tiezhong ^{[1
]}

Liu, Yingzi ^{[1
]}

Gao, Shenyang ^{[1
]}

Li, Li ^{[1
]}

Deng, Xuming ^{[4
]}

机构：

[1] Liaoning Med Univ, Inst Anim Husb & Vet Med, Jinzhou 121001, Liaoning, Peoples R China

[2] Chinanim Agr Dev Co Ltd, Beijing 100070, Peoples R China

[3] Nanjing Shengyi Pharmacol Technol LLC, Nanjing 210000, Jiangsu, Peoples R China

[4] Jilin Univ, Coll Anim Husb & Vet Med, Changchun 130062, Jilin, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 02期

关键词：

Kadsura marmorata; Response surface methodology; Polysaccharide; Extraction; Optimization; Characterization; RESPONSE-SURFACE METHODOLOGY; OPTIMIZATION; DESIGN;

D O I：

10.1016/j.carbpol.2012.11.060

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, response surface methodology and central composite design based on single-factor experiments were applied to the optimization of the extraction parameters (extraction time, extraction temperature, and solid-liquid ratio) of polysaccharides from Kadsura marmorata fruits. The extraction temperature and solid-liquid ratio affected the extraction yield significantly. The optimal conditions included a solid-liquid ratio of 1:27 (mass/volume), an extraction time of 3 h and an extraction temperature of 98 degrees C. Under these conditions, the maximal yield of crude Kadsura polysaccharide (KPS) was 2.7735 +/- 0.068%, which agreed with model predictions. Five major fractions, KPS I, KPS II, KPS III, KPS IV and KPS V, were obtained by diethylaminoethyl (DEAE) cellulose-52 column chromatography. The main fraction, KPS III-1, was isolated from KPS III by Sephadex G-100 column chromatography. Preliminary characterization of KPS III-1 by gas chromatography indicated that it was composed mainly of xylose and galacturonic acid. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

引用

页码：1901 / 1907

页数：7

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