Least squares spectral resolution of liquid chromatography-mass spectrometry data of glycerophospholipids

被引：3

作者：

Zeng, Ying-Xu ^{[1
]}

Mjos, Svein A. ^{[1
]}

Meier, Sonnich ^{[2
]}

Lin, Chen-Chen ^{[1
]}

Vadla, Reidun ^{[1
,2
]}

机构：

[1] Univ Bergen, Dept Chem, N-5020 Bergen, Norway

[2] Inst Marine Res, NO-5817 Bergen, Norway

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1280卷

关键词：

Glycerophospholipids; Phosphatidylcholine; Phosphatidylethanolamine; Sphingomyelins; LC-MS; Least squares spectral resolution; HIGH-PERFORMANCE LIQUID; COD GADUS-MORHUA; ELECTROSPRAY-IONIZATION; QUANTITATIVE-ANALYSIS; MOUSE-BRAIN; FATTY-ACID; MULTIPLE PRECURSOR; BIOLOGICAL SAMPLES; IDENTIFICATION; PHOSPHATIDYLCHOLINE;

D O I：

10.1016/j.chroma.2012.12.070

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatography-mass spectrometry represents a powerful tool for the analysis of intact glycerophospholipids (GPLs), but manual data interpretation may be a bottleneck in these analyses. The present paper proposes a least square regression approach for the automated characterization and deconvolution of the main GPLs species, i.e., phosphatidylcholine and phosphatidylethanolamine analyzed by class-specific scanning methods such as precursor ion scanning and neutral loss scanning, respectively. The algorithm is based on least squares resolution of spectra and chromatograms from theoretically calculated mass spectra, and eliminates the need for isotope correction. Results from the application of the methodology on reference compounds and extracts of cod brain and mouse brain are presented. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：23 / 34

页数：12

共 46 条

[1]

[Anonymous], 1978, J Lipid Res, V19, P114

[2] MOLECULAR-SPECIES COMPOSITION OF THE MAJOR DIACYL GLYCEROPHOSPHOLIPIDS FROM MUSCLE, LIVER, RETINA AND BRAIN OF COD (GADUS-MORHUA) [J].