Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display

被引：40
|
作者：
Truesdell, GM
Dickman, MB
机构：
[1] UNIV NEBRASKA,DEPT PLANT PATHOL,LINCOLN,NE 68583
[2] UNIV NEBRASKA,SCH BIOL SCI,LINCOLN,NE 68583
来源：
PLANT MOLECULAR BIOLOGY | 1997年 / 33卷 / 04期
关键词：
alfalfa; differential display; gene family; plant defense; plant stress; wound-induced gene expression;
D O I：
10.1023/A:1005728420374
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Differential display of mRNA was used to isolate a full-length (SRG1) and a partial (SRG2) alfalfa cDNA induced during infection with the fungal pathogen Colletotrichum trifolii. The deduced amino acid sequences are similar to each other and resemble plant defense-related proteins and tree pollen allergens. SRG1 is a member of a gene family in alfalfa, which may also include the putative defense-related gene PR10. Unlike many defense-related genes described in similar systems, expression of SRG1-like genes does not correlate with resistance to C. trifolii. We speculate SRG1 is induced in response to plant stress.
引用
收藏
页码：737 / 743
页数：7
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