共 53 条
13C APSY-NMR for sequential assignment of intrinsically disordered proteins
被引:16
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Sainati, Valerio
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Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy

Bermel, Wolfgang
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机构:
Bruker BioSpin GmbH, D-76287 Silberstreifen, Rheinstetten, Germany Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy

Pierattelli, Roberta
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机构:
Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy

Felli, Isabella C.
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Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy
机构:
[1] Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy
[2] Bruker BioSpin GmbH, D-76287 Silberstreifen, Rheinstetten, Germany
[3] Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy
关键词:
Intrinsically disordered proteins;
IDPs;
Assignment;
NUS;
C-13;
detection;
AUTOMATED PROJECTION SPECTROSCOPY;
MULTIDIMENSIONAL NMR;
RECONSTRUCTION;
EXTENSION;
DYNAMICS;
D O I:
10.1007/s10858-018-0167-4
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The increasingly recognized biological relevance of intrinsically disordered proteins requires a continuous expansion of the tools for their characterization via NMR spectroscopy, the only technique so far able to provide atomic-resolution information on these highly mobile macromolecules. Here we present the implementation of projection spectroscopy in C-13-direct detected NMR experiments to achieve the sequence specific assignment of IDPs. The approach was used to obtain the complete backbone assignment at high temperature of alpha-synuclein, a paradigmatic intrinsically disordered protein.
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页码:167 / 175
页数:9
相关论文
共 53 条
[1]
Targeting intrinsically disordered proteins in rational drug discovery
[J].
Ambadipudi, Susmitha
;
Zweckstetter, Markus
.
EXPERT OPINION ON DRUG DISCOVERY,
2016, 11 (01)
:65-77

Ambadipudi, Susmitha
论文数: 0 引用数: 0
h-index: 0
机构:
German Ctr Neurodegenerat Dis DZNE, D-37077 Gottingen, Germany German Ctr Neurodegenerat Dis DZNE, D-37077 Gottingen, Germany

Zweckstetter, Markus
论文数: 0 引用数: 0
h-index: 0
机构:
German Ctr Neurodegenerat Dis DZNE, D-37077 Gottingen, Germany
Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany
Univ Med Ctr Groningen, DFG Res Ctr Nanoscale Microscopy & Mol Physiol Br, D-37073 Gottingen, Germany German Ctr Neurodegenerat Dis DZNE, D-37077 Gottingen, Germany
[2]
13C-detected protonless NMR spectroscopy of proteins in solution
[J].
Bermel, W
;
Bertini, I
;
Felli, IC
;
Piccioli, M
;
Pierattelli, R
.
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY,
2006, 48 (01)
:25-45

Bermel, W
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Bertini, I
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Felli, IC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Piccioli, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

Pierattelli, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
[3]
Protonless NMR experiments for sequence-specific assignment of backbone nuclei in unfolded proteins
[J].
Bermel, W
;
Bertini, I
;
Felli, IC
;
Lee, YM
;
Luchinat, C
;
Pierattelli, R
.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
2006, 128 (12)
:3918-3919

Bermel, W
论文数: 0 引用数: 0
h-index: 0
机构: Bruker BioSpin GmbH, Rheinstetten, Germany

Bertini, I
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, Rheinstetten, Germany Bruker BioSpin GmbH, Rheinstetten, Germany

Felli, IC
论文数: 0 引用数: 0
h-index: 0
机构: Bruker BioSpin GmbH, Rheinstetten, Germany

Lee, YM
论文数: 0 引用数: 0
h-index: 0
机构: Bruker BioSpin GmbH, Rheinstetten, Germany

Luchinat, C
论文数: 0 引用数: 0
h-index: 0
机构: Bruker BioSpin GmbH, Rheinstetten, Germany

Pierattelli, R
论文数: 0 引用数: 0
h-index: 0
机构: Bruker BioSpin GmbH, Rheinstetten, Germany
[4]
Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins
[J].
Bermel, W
;
Bertini, I
;
Felli, IC
;
Kümmerle, R
;
Pierattelli, R
.
JOURNAL OF MAGNETIC RESONANCE,
2006, 178 (01)
:56-64

Bermel, W
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy

Bertini, I
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy

Felli, IC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy

Kümmerle, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy

Pierattelli, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy
[5]
13C direct-detection biomolecular NMR
[J].
Bermel, Wolfgang
;
Felli, Isabella C.
;
Kuemmerle, Rainer
;
Pierattelli, Roberta
.
CONCEPTS IN MAGNETIC RESONANCE PART A,
2008, 32A (03)
:183-200

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Silberstreifen, Rheinstetten, Germany Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy
Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

Kuemmerle, Rainer
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin AG, CH-8117 Fallanden, Switzerland Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy
Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy
[6]
High-dimensionality 13C direct-detected NMR experiments for the automatic assignment of intrinsically disordered proteins
[J].
Bermel, Wolfgang
;
Felli, Isabella C.
;
Gonnelli, Leonardo
;
Kozminski, Wiktor
;
Piai, Alessandro
;
Pierattelli, Roberta
;
Zawadzka-Kazimierczuk, Anna
.
JOURNAL OF BIOMOLECULAR NMR,
2013, 57 (04)
:353-361

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Sesto Fiorentino, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Gonnelli, Leonardo
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Kozminski, Wiktor
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Warsaw, Fac Chem, Biol & Chem Res Ctr, PL-02089 Warsaw, Poland Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Piai, Alessandro
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Sesto Fiorentino, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

论文数: 引用数:
h-index:
机构:
[7]
Improving the chemical shift dispersion of multidimensional NMR spectra of intrinsically disordered proteins
[J].
Bermel, Wolfgang
;
Bruix, Marta
;
Felli, Isabella C.
;
Kumar, Vasantha M. V.
;
Pierattelli, Roberta
;
Serrano, Soraya
.
JOURNAL OF BIOMOLECULAR NMR,
2013, 55 (03)
:231-237

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Bruix, Marta
论文数: 0 引用数: 0
h-index: 0
机构:
CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Dept Chem Ugo Shiff, I-50019 Florence, Italy
Univ Florence, Magnet Resonance Ctr, I-50019 Florence, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Kumar, Vasantha M. V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Magnet Resonance Ctr, I-50019 Florence, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, Dept Chem Ugo Shiff, I-50019 Florence, Italy
Univ Florence, Magnet Resonance Ctr, I-50019 Florence, Italy Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany

Serrano, Soraya
论文数: 0 引用数: 0
h-index: 0
机构:
CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany
[8]
Speeding up sequence specific assignment of IDPs
[J].
Bermel, Wolfgang
;
Bertini, Ivano
;
Felli, Isabella C.
;
Gonnelli, Leonardo
;
Kozminski, Wiktor
;
Piai, Alessandro
;
Pierattelli, Roberta
;
Stanek, Jan
.
JOURNAL OF BIOMOLECULAR NMR,
2012, 53 (04)
:293-301

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, Silberstreifen, D-76287 Rheinstetten, Germany Univ Florence, CERM, I-50019 Florence, Italy

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Gonnelli, Leonardo
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Kozminski, Wiktor
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland Univ Florence, CERM, I-50019 Florence, Italy

Piai, Alessandro
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

论文数: 引用数:
h-index:
机构:
[9]
Speeding Up 13C Direct Detection Biomolecular NMR Spectroscopy
[J].
Bermel, Wolfgang
;
Bertini, Ivano
;
Felli, Isabella C.
;
Pierattelli, Roberta
.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
2009, 131 (42)
:15339-15345

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany Univ Florence, CERM, I-50019 Florence, Italy

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, I-50019 Florence, Italy
Univ Florence, Dept Chem, I-50019 Florence, Italy Univ Florence, CERM, I-50019 Florence, Italy
[10]
H-start for exclusively heteronuclear NMR spectroscopy: The case of intrinsically disordered proteins
[J].
Bermel, Wolfgang
;
Bertini, Ivano
;
Csizmok, Veronika
;
Felli, Isabella C.
;
Pierattelli, Roberta
;
Tompa, Peter
.
JOURNAL OF MAGNETIC RESONANCE,
2009, 198 (02)
:275-281

Bermel, Wolfgang
论文数: 0 引用数: 0
h-index: 0
机构:
Bruker BioSpin GmbH, D-76287 Rheinstetten, Germany Univ Florence, CERM, IT-50019 Florence, Italy

Bertini, Ivano
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, IT-50019 Florence, Italy
Univ Florence, Dept Chem, IT-50019 Florence, Italy Univ Florence, CERM, IT-50019 Florence, Italy

Csizmok, Veronika
论文数: 0 引用数: 0
h-index: 0
机构:
Hungarian Acad Sci, Biol Res Ctr, Inst Enzymol, H-1113 Budapest, Hungary Univ Florence, CERM, IT-50019 Florence, Italy

Felli, Isabella C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, IT-50019 Florence, Italy
Univ Florence, Dept Chem, IT-50019 Florence, Italy Univ Florence, CERM, IT-50019 Florence, Italy

Pierattelli, Roberta
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Florence, CERM, IT-50019 Florence, Italy
Univ Florence, Dept Chem, IT-50019 Florence, Italy Univ Florence, CERM, IT-50019 Florence, Italy

Tompa, Peter
论文数: 0 引用数: 0
h-index: 0
机构:
Hungarian Acad Sci, Biol Res Ctr, Inst Enzymol, H-1113 Budapest, Hungary Univ Florence, CERM, IT-50019 Florence, Italy