13C APSY-NMR for sequential assignment of intrinsically disordered proteins

被引:16
作者
Murrali, Maria Grazia [1 ]
Schiavina, Marco [1 ]
Sainati, Valerio [1 ]
Bermel, Wolfgang [2 ]
Pierattelli, Roberta [1 ,3 ]
Felli, Isabella C. [1 ,3 ]
机构
[1] Univ Florence, CERM, Via Luigi Sacconi 6, I-50019 Florence, Italy
[2] Bruker BioSpin GmbH, D-76287 Silberstreifen, Rheinstetten, Germany
[3] Univ Florence, Dept Chem Ugo Schiff, I-50019 Florence, Italy
关键词
Intrinsically disordered proteins; IDPs; Assignment; NUS; C-13; detection; AUTOMATED PROJECTION SPECTROSCOPY; MULTIDIMENSIONAL NMR; RECONSTRUCTION; EXTENSION; DYNAMICS;
D O I
10.1007/s10858-018-0167-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The increasingly recognized biological relevance of intrinsically disordered proteins requires a continuous expansion of the tools for their characterization via NMR spectroscopy, the only technique so far able to provide atomic-resolution information on these highly mobile macromolecules. Here we present the implementation of projection spectroscopy in C-13-direct detected NMR experiments to achieve the sequence specific assignment of IDPs. The approach was used to obtain the complete backbone assignment at high temperature of alpha-synuclein, a paradigmatic intrinsically disordered protein.
引用
收藏
页码:167 / 175
页数:9
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