Inflammatory Breast Carcinoma: Elevated microRNA miR-181b-5p and Reduced miR-200b-3p, miR-200c-3p, and miR-203a-3p Expression as Potential Biomarkers with Diagnostic Value

被引:25
|
作者
Fahim, Sarah Atef [1 ]
Abdullah, Mahmoud Salah [2 ]
Espinoza-Sanchez, Nancy A. [3 ]
Hassan, Hebatallah [4 ]
Ibrahim, Ayman M. [4 ]
Ahmed, Sarah Hamdy [2 ]
Shakir, George [2 ,5 ]
Badawy, Mohamed A. [6 ]
Zakhary, Nadia, I [7 ]
Greve, Burkhard [8 ]
El-Shinawi, Mohamed [9 ]
Gotte, Martin [3 ]
Ibrahim, Sherif Abdelaziz [4 ]
机构
[1] Cairo Univ, Fac Sci, Chem Dept, Biochem Program, Giza 12613, Egypt
[2] Cairo Univ, Fac Sci, Chem Dept, Biotechnol Biomol Chem Program, Giza 12613, Egypt
[3] Munster Univ Hosp, Dept Gynecol & Obstet, D-48149 Munster, Germany
[4] Cairo Univ, Fac Sci, Dept Zool, Giza 12613, Egypt
[5] Ludwig Maximilians Univ Munchen, Inst Cardiovasc Prevent, D-80539 Munich, Germany
[6] Cairo Univ, Fac Sci, Chem Dept, Giza 12613, Egypt
[7] Cairo Univ, Natl Canc Inst, Canc Biol Dept, Cairo 11796, Egypt
[8] Univ Hosp Munster, Dept Radiotherapy Radiooncol, D-48149 Munster, Germany
[9] Ain Shams Univ, Fac Med, Dept Gen Surg, Cairo 11566, Egypt
关键词
inflammatory breast cancer; microRNAs; miR-181b-5p; miR-200b-3p; miR-200c-3p; miR-203a-3p; miR-1-3p; ZEB2; hub genes; EPITHELIAL-MESENCHYMAL TRANSITION; THERAPEUTIC TARGETS; REPRESSORS ZEB1; CELL-MIGRATION; CANCER-CELLS; FAMILY; PROLIFERATION; SURVIVAL; MIR-1; METASTASIS;
D O I
10.3390/biom10071059
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inflammatory breast cancer (IBC) is a rare yet aggressive breast cancer variant, associated with a poor prognosis. The major challenge for IBC is misdiagnosis due to the lack of molecular biomarkers. We profiled dysregulated expression of microRNAs (miRNAs) in primary samples of IBC and non-IBC tumors using human breast cancer miRNA PCR array. We discovered that 28 miRNAs were dysregulated (10 were upregulated, while 18 were underexpressed) in IBC vs. non-IBC tumors. We identified 128 hub genes, which are putative targets of the differentially expressed miRNAs and modulate important cancer biological processes. Furthermore, our qPCR analysis independently verified a significantly upregulated expression of miR-181b-5p, whereas a significant downregulation of miR-200b-3p, miR-200c-3p, and miR-203a-3p was detected in IBC tumors. Receiver operating characteristic (ROC) curves implied that the four miRNAs individually had a diagnostic accuracy in discriminating patients with IBC from non-IBC and that miR-203a-3p had the highest diagnostic value with an AUC of 0.821. Interestingly, a combination of miR-181b-5p, miR-200b-3p, and miR-200c-3p robustly improved the diagnostic accuracy, with an area under the curve (AUC) of 0.897. Intriguingly, qPCR revealed that the expression of zinc finger E box-binding homeobox 2 (ZEB2) mRNA, the putative target of miR-200b-3p, miR-200c-3p, and miR-203a-3p, was upregulated in IBC tumors. Overall, this study identified a set of miRNAs serving as potential biomarkers with diagnostic relevance for IBC.
引用
收藏
页码:1 / 30
页数:30
相关论文
共 50 条
  • [1] HMGB3 promotes the proliferation and metastasis of glioblastoma and is negatively regulated by miR-200b-3p and miR-200c-3p
    Liu, Jianxun
    Wang, Liling
    Li, Xuesong
    CELL BIOCHEMISTRY AND FUNCTION, 2018, 36 (07) : 357 - 365
  • [2] miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study
    Kacper Pełka
    Klaudia Klicka
    Tomasz M. Grzywa
    Agata Gondek
    Janina M. Marczewska
    Filip Garbicz
    Kinga Szczepaniak
    Wiktor Paskal
    Paweł K. Włodarski
    Histochemistry and Cell Biology, 2021, 155 : 423 - 433
  • [3] miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia-archival samples study
    Pelka, Kacper
    Klicka, Klaudia
    Grzywa, Tomasz M.
    Gondek, Agata
    Marczewska, Janina M.
    Garbicz, Filip
    Szczepaniak, Kinga
    Paskal, Wiktor
    Wlodarski, Pawel K.
    HISTOCHEMISTRY AND CELL BIOLOGY, 2021, 155 (03) : 423 - 433
  • [4] Circulating miR-23b-3p, miR-145-5p and miR-200b-3p are potential biomarkers to monitor acute pain associated with laminitis in horses
    Lecchi, C.
    Dalla Costa, E.
    Lebelt, D.
    Ferrante, V.
    Canali, E.
    Ceciliani, F.
    Stucke, D.
    Minero, M.
    ANIMAL, 2018, 12 (02) : 366 - 375
  • [5] The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors
    Gyvyte, Ugne
    Lukosevicius, Rokas
    Inciuraite, Ruta
    Streleckiene, Greta
    Gudoityte, Greta
    Bekampyte, Justina
    Valentini, Serena
    Salteniene, Violeta
    Ruzgys, Paulius
    Satkauskas, Saulius
    Zviniene, Kristina
    Kupcinskas, Juozas
    Skieceviciene, Jurgita
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (14) : 1 - 12
  • [6] Dysregulation of miR-21-5p, miR-93-5p, miR-200c-3p and miR-205-5p in Oral Squamous Cell Carcinoma: A Potential Biomarkers Panel?
    Aghiorghiesei, Ovidiu
    Zanoaga, Oana
    Raduly, Lajos
    Aghiorghiesei, Alexandra Iulia
    Chiroi, Paul
    Trif, Andrada
    Buiga, Rares
    Budisan, Liviuta
    Lucaciu, Ondine
    Pop, Laura Ancuta
    Braicu, Cornelia
    Campian, Radu
    Berindan-Neagoe, Ioana
    CURRENT ISSUES IN MOLECULAR BIOLOGY, 2022, 44 (04) : 1754 - 1767
  • [7] Expression of human miR-200b-3p and-200c-3p in cytomegalovirus-infected tissues
    Lee, Kyoung Hwa
    Lim, Beom Jin
    Ferreira, Victor H.
    Min, Seo Yeon
    Hong, Yeon-Mi
    Jo, Jeong-Hyeon
    Han, Sang Hoon
    BIOSCIENCE REPORTS, 2018, 38
  • [8] miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children
    Visani, Michela
    Marucci, Gianluca
    de Biase, Dario
    Giangaspero, Felice
    Buttarelli, Francesca Romana
    Brandes, Alba Ariela
    Franceschi, Enrico
    Acquaviva, Giorgia
    Ciarorcchi, Alessia
    Rhoden, Kerry Jane
    Tallini, Giovanni
    Pession, Annalisa
    DIAGNOSTICS, 2020, 10 (05)
  • [9] Diagnostic panel of serum miR-125b-5p, miR-182-5p, and miR-200c-3p as non-invasive biomarkers for urothelial bladder cancer
    Wen, Z.
    Huang, G.
    Lai, Y.
    Xiao, L.
    Peng, X.
    Liu, K.
    Zhang, C.
    Chen, X.
    Li, R.
    Li, X.
    Lai, Y.
    Ni, L.
    CLINICAL & TRANSLATIONAL ONCOLOGY, 2022, 24 (05): : 909 - 918
  • [10] Diagnostic panel of serum miR-125b-5p, miR-182-5p, and miR-200c-3p as non-invasive biomarkers for urothelial bladder cancer
    Z. Wen
    G. Huang
    Y. Lai
    L. Xiao
    X. Peng
    K. Liu
    C. Zhang
    X. Chen
    R. Li
    X. Li
    Y. Lai
    L. Ni
    Clinical and Translational Oncology, 2022, 24 : 909 - 918