Cap-independent translation initiation of Apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5′ untranslated region

被引：15
作者：
Andreev, D. E. [1 ]
Dmitriev, S. E. [1 ]
Terenin, I. M. [1 ]
Shatsky, I. N. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119991, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2013年 / 78卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
protein biosynthesis; translational control; cellular IRES-elements; cap-independent translation; Apaf-1; mRNA; INTERNAL RIBOSOME ENTRY; IRES-MEDIATED TRANSLATION; DEPENDENT TRANSLATION; STRESS; SITE; APOPTOSIS; SEGMENT; EIF4G;
D O I：
10.1134/S0006297913020041
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have earlier shown that the 5'-untranslated region (5' UTR) of the mRNA coding for activation factor of apoptotic peptidase 1 (Apaf-1) can direct translation in vivo by strictly 5' end-dependent way even in the absence of m(7)G-cap. Dependence of translational efficiency on the cap availability for this mRNA turned out to be relatively low. In this study we demonstrate that this surprising phenomenon is determined the 5'-proximal part (domains I and II) of highly structured Apaf-1 5' UTR. Remarkably, domain II by itself was able to reduce dependence of the mRNA on the cap on its transferring to a short 5' UTR derived from a standard vector. We suggest that the low cap-dependence inherent to some cellular mRNAs may have an important physiological significance under those stress conditions when the function of cap-binding factor eIF4E is impaired.
引用
收藏
页码：157 / 165
页数：9
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