Development and Application of CRISPR/Cas System in Rice

被引：8

作者：

Ren Jun ^{[1
]}

Hu Xixun ^{[1
]}

Wang Kejian ^{[1
]}

Wang Chun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

来源：

RICE SCIENCE | 2019年 / 26卷 / 02期

基金：

中国国家自然科学基金;

关键词：

rice; CRISPR; Cas; genome editing; protospacer adjacent motif; TARGETED MUTAGENESIS; GENOMIC DNA; PLANTS; CRISPR-CAS9; NUCLEASES; CPF1; BASE; ENDONUCLEASE; RECOGNITION; GENERATION;

D O I：

10.1016/j.rsci.2019.01.001

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In the past several years, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system has been harnessed as an efficient and powerful tool for targeted genome editing in different prokaryotic and eukaryotic species. Here, we review the development and application of CRISPR/Cas system in rice, emphasizing different varieties of CRISPR/Cas systems have been applied, strategies for multiplex editing, methods for precise gene insertion and replacement, and efficient systems for base editing and site-specific transcriptional regulation. In addition, the biosecurity of CRISPR/Cas system is also discussed, including transgene-free methods and off-target effects of CRISPR/Cas system. Thus, the development and application of CRISPR/Cas system will have significant impact on functional genomic research and variety improvement in rice.

引用

页码：69 / 76

页数：8

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