Fosmid cloning, nucleotide sequence, and characterization of a beta-lactamase gene from subsurface isolates

被引:0
|
作者
Vardar, Nurcan [1 ]
Vardar-Schara, Gonul [1 ]
机构
[1] Fatih Univ, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey
基金
美国农业部;
关键词
beta-lactamase; terrestrial subsurface; antibiotic resistance; direct cloning; BACILLUS-LICHENIFORMIS; ANTIBIOTIC-RESISTANCE;
D O I
10.1007/s12275-012-2139-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A beta-lactamase gene was isolated for the first time from a terrestrial subsurface environment using a combined cultivation and direct cloning strategy. The gene, discovered from 24 m below land surface in Hawaii, was most similar to the penicillinase from Bacillus licheniformis. The resistance gene was confirmed via subcloning and its minimum inhibitory concentration values were measured against several test beta-lactam antibiotics. This study extends the knowledge on resistance to antimicrobials, which may help the efforts to minimize their future threat.
引用
收藏
页码:680 / 683
页数:4
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