Inhibitory effect of Piper betel leaf extracts on copper-mediated LDL oxidation and oxLDL-induced lipid accumulation via inducing reverse cholesterol transport in macrophages

被引：17

作者：

Ma, Gwo-Chin ^{[1
,2
]}

Wu, Pei-Fang ^{[1
]}

Tseng, Hsien-Chun ^{[3
,4
]}

Chyau, Charng-Cherng ^{[5
]}

Lu, Hsiu-Chin ^{[6
]}

Chou, Fen-Pi ^{[1
]}

机构：

[1] Chung Shan Med Univ, Coll Med, Dept Inst Biochem & Biotechnol, Taichung 402, Taiwan

[2] Changhua Christian Hosp, Dept Genom Med, Changhua, Taiwan

[3] Chung Shan Med Univ Hosp, Dept Radiat Oncol, Taichung, Taiwan

[4] Chung Shan Med Univ, Coll Med Sci & Technol, Sch Med Imaging & Radiol Sci, Taichung, Taiwan

[5] HungKuang Univ, Biotechnol Res Inst, Taichung, Taiwan

[6] China Med Univ Hosp, Dept Lab Med, Taichung, Taiwan

来源：

FOOD CHEMISTRY | 2013年 / 141卷 / 04期

关键词：

Atherosclerosis; Oxidised low density lipoprotein; Piper betel leaf; Reverse cholesterol transport; FOAM CELL-FORMATION; OXIDIZED LDL; SR-A; EXPRESSION; ANTIOXIDANT; L; CYTOTOXICITY; ACTIVATION; MECHANISMS; FLAVONOIDS;

D O I：

10.1016/j.foodchem.2013.06.003

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Piper betel leaf (PBL) has the biological capabilities of detoxification and can work as an anti-inflammatory agent and an anti-oxidant. In this study, we evaluated the anti-oxidative activity of the extract of Piper betel leaves (PBLs) on the basis of Cu2+-mediated oxidation, and its ability to prevent foam cell formation in a model for oxidised low density lipoprotein (oxLDL)-induced lipid accumulation in macrophages. Our data demonstrated that PBLs were able to inhibit LDL oxidation in vitro and are able to reduce the lipid accumulation in macrophages. We showed the underlying mechanisms to be the following: PBLs up-regulated the protein levels of the class A and class B scavenger receptors, the membrane lipid transporter ABCA1, and its upstream regulator Liver X receptor (LXR) in the macrophages exposed to oxLDL. The results suggested that PBLs activated the reverse cholesterol transport mechanism to enhance the metabolism of the oxLDL that could prevent both lipid accumulation and foam cell formation and further minimise the possible damage of vessels caused by the oxLDL. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：3703 / 3713

页数：11

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