Simultaneous determination of three carbapenem antibiotics in plasma by HPLC with ultraviolet detection

被引：88

作者：

Legrand, Tiphaine ^{[1
]}

Chhun, Stephanie ^{[1
]}

Rey, Elisabeth ^{[1
]}

Blanchet, Benoit ^{[2
]}

Zahar, Jean-Ralph ^{[3
]}

Lanternier, Fanny ^{[4
]}

Pons, Gerard ^{[1
]}

Jullien, Vincent ^{[1
]}

机构：

[1] Univ Paris 05, Grp Hosp Cochin St Vincent de Paul, AP HP, INSERM,Serv Pharmacol Clin,U663, F-75014 Paris, France

[2] Grp Hosp Cochin St Vincent de Paul, AP HP, Serv Pharm, F-75014 Paris, France

[3] Univ Paris 05, Hop Necker Enfants Malad, AP HP, Equipe Mobile Infectiol,Serv Microbiol Hyg Hosp, F-75015 Paris, France

[4] Univ Paris 05, Hop Necker Enfants Malad, AP HP, Serv Malad Infect & Trop, F-75015 Paris, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 02期

关键词：

Imipenem; Meropenem; Ertapenem; Carbapenem; HPLC; Ultraviolet detection;

D O I：

10.1016/j.jchromb.2008.09.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, precise and accurate high-performance liquid chromatography (HPLC) method using ultraviolet (UV) detection has been developed for simultaneous determination of carbapenem antibiotics: imipenem, meropenem and ertapenem in human plasma. Samples were spiked with ceftazidime as internal standard and proteins were precipitated by acetonitrile. Separation was achieved on a C8 column with a mobile phase composed of phosphate buffer 0.1 M (pH 6.8) and methanol in gradient elution mode. Detection was performed at 298 nm. Calibration curves were linear from 0.5 to 80 mg/L for each compound, with Correlation coefficients over 0.997. Intra- and inter-day validation studies showed accuracy between -4.5 and 8.1% and precision below 10.4%. Mean recoveries were 82.2,90.8 and 87.7% for imipenem, meropenem and ertapenem. respectively. This method provides a useful tool for the therapeutic drug monitoring of carbapenems. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：551 / 556

页数：6

共 36 条

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