Optimisation of a direct plating method for the detection and enumeration of Alicyclobacillus acidoterrestris spores

被引：15
作者：
Henczka, Marek [1 ]
Djas, Malgorzata [1 ]
Filipek, Katarzyna [1 ]
机构：
[1] Warsaw Univ Technol, Fac Chem & Proc Engn, PL-00645 Warsaw, Poland
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 92卷 / 01期
关键词：
Alicyclobacillus acidoterrestris; Detection method; Growth medium; Membrane filtration; Spores; SP NOV; FORMING BACTERIUM; FATTY-ACIDS; FRUIT JUICE; GROWTH; SPP;
D O I：
10.1016/j.mimet.2012.10.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A direct plating method for the detection and enumeration of Alicyclobacillus acidoterrestris spores has been optimised. The results of the application of four types of growth media (BAT agar, YSG agar, K agar and SK agar) regarding the recovery and enumeration of A. acidoterrestris spores were compared. The influence of the type of applied growth medium, heat shock conditions, incubation temperature, incubation time, plating technique and the presence of apple juice in the sample on the accuracy of the detection and enumeration of A. acidoterrestris spores was investigated. Among the investigated media, YSG agar was the most sensitive medium, and its application resulted in the highest recovery of A. acidoterrestris spores, while K agar and BAT agar were the least suitable media. The effect of the heat shock time on the recovery of spores was negligible. When there was a low concentration of spores in a sample, the membrane filtration method was superior to the spread plating method. The obtained results show that heat shock carried out at 80 degrees C for 10 min and plating samples in combination with membrane filtration on YSG agar, followed by incubation at 46 degrees C for 3 days provided the optimal conditions for the detection and enumeration of A. acidoterrestris spores. Application of the presented method allows highly efficient, fast and sensitive identification and enumeration of A. acidoterrestris spores in food products. This methodology will be useful for the fruit juice industry for identifying products contaminated with A. acidoterrestris spores, and its practical application may prevent economic losses for manufacturers. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：1 / 8
页数：8
相关论文
共 28 条
[11]  
Jensen N, 1999, FOOD AUST, V51, P33
[12]  
Jensen N, 2000, FOOD AUST, V52, P282
[13]  
Jensen N., 2005, 14 INT FED FRUIT JUI
[14]   Membrane filtration method for enumeration and isolation of Alicyclobacillus spp. from apple juice [J].
Lee, S.-Y. ;
Chang, S.-S. ;
Shin, J.-H. ;
Kang, D.-H. .
LETTERS IN APPLIED MICROBIOLOGY, 2007, 45 (05) :540-546
[15]  
Matsubara H, 2002, INT J SYST EVOL MICR, V52, P1681, DOI 10.1099/00207713-52-5-1681
[16]   Evaluation of direct plating methods to enumerate Alicyclobacillus in beverages [J].
Murray, Melinda. B. ;
Gurtler, Joshua B. ;
Ryu, Jee-Hoon ;
Harrison, Mark A. ;
Beuchat, Larry R. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 115 (01) :59-69
[17]   Methods for the detection and enumeration of Alicyclobacillus acidoterrestris and investigation of growth and production of taint in fruit juice and fruit juice-containing drinks [J].
Pettipher, GL ;
Osmundson, ME ;
Murphy, JM .
LETTERS IN APPLIED MICROBIOLOGY, 1997, 24 (03) :185-189
[18]  
Pettipher GL, 2000, FOOD AUST, V52, P293
[19]   Alicyclobacillus acidoterrestris spores in fruit products and design of pasteurization processes [J].
Silva, FVM ;
Gibbs, P .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2001, 12 (02) :68-74
[20]   Influence of Different Filling, Cooling, and Storage Conditions on the Growth of Alicyclobacillus acidoterrestris CRA7152 in Orange Juice [J].
Spinelli, Ana Claudia N. F. ;
Sant'Ana, Anderson S. ;
Rodrigues-Junior, Salatir ;
Massaguer, Pilar R. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (23) :7409-7416
123