Existence of uncoupling protein-2 antigen in isolated mitochondria from various tissues

被引：12
作者：
Jezek, P
Zácková, M
Reháková, Z
Ruzicka, M
Borecky, J
Skobisová, E
Brucknerová, J
Garlid, KD
Gimeno, RE
Tartaglia, LA
机构：
[1] Inst Physiol, Dept Membrane Transport Biophys, CZ-14220 Prague, Czech Republic
[2] Inst Microbiol, Dept Gnotobiol, Novy Hradek, Czech Republic
[3] Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, Portland, OR 97291 USA
[4] Millennium Pharmaceut Inc, Cambridge, MA 02139 USA
来源：
FEBS LETTERS | 1999年 / 455卷 / 1-2期
关键词：
anti-UCP3; antibody; uncoupling protein 2; uncoupling protein 3;
D O I：
10.1016/S0014-5793(99)00853-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Antibodies against Escherichia coli-expressed uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs, The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein-1 (UCP1) isolated from brown adipose tissue. Moreover, anti-UCP2 and cross-reacting anti-UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：79 / 82
页数：4
相关论文
共 33 条
[1]   ADIPOSE CELL ORGANELLES - ISOLATION MORPHOLOGY AND POSSIBLE RELATION TO INTRACELLULAR LIPID TRANSPORT [J].
ANGEL, A ;
SHELDON, H .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1965, 131 (A1) :157-+
[2]   The uncoupling proteins, a review [J].
Boss, O ;
Muzzin, P ;
Giacobino, JP .
EUROPEAN JOURNAL OF ENDOCRINOLOGY, 1998, 139 (01) :1-9
[3]   Effect of endurance training on mRNA expression of uncoupling proteins 1, 2, and 3 in the rat [J].
Boss, O ;
Samec, S ;
Desplanches, D ;
Mayet, MH ;
Seydoux, J ;
Muzzin, P ;
Giacobino, JP .
FASEB JOURNAL, 1998, 12 (03) :335-339
[4]   Tissue-dependent upregulation of rat uncoupling protein-2 expression in response to fasting or cold [J].
Boss, O ;
Samec, S ;
Dulloo, A ;
Seydoux, J ;
Muzzin, P ;
Giacobino, JP .
FEBS LETTERS, 1997, 412 (01) :111-114
[5]   Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression [J].
Boss, O ;
Samec, S ;
PaoloniGiacobino, A ;
Rossier, C ;
Dulloo, A ;
Seydoux, J ;
Muzzin, P ;
Giacobino, JP .
FEBS LETTERS, 1997, 408 (01) :39-42
[6]   Uncoupling protein-3 expression in rodent skeletal muscle is modulated by food intake but not by changes in environmental temperature [J].
Boss, O ;
Samec, S ;
Kühne, F ;
Bijlenga, P ;
Assimacopoulos-Jeannet, F ;
Seydoux, J ;
Giacobino, JP ;
Muzzin, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (01) :5-8
[7]   Differential regulation of uncoupling protein-2 and uncoupling protein-3 gene expression in brown adipose tissue during development and cold exposure [J].
Carmona, MC ;
Valmaseda, A ;
Brun, S ;
Viñas, O ;
Mampel, T ;
Iglesias, R ;
Giralt, M ;
Villarroya, F .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 243 (01) :224-228
[8]   Obesity induces expression of uncoupling protein-2 in hepatocytes and promotes liver ATP depletion [J].
Chavin, KD ;
Yang, SQ ;
Lin, HZ ;
Chatham, J ;
Chacko, VP ;
Hoek, JB ;
Walajtys-Rode, E ;
Rashid, A ;
Chen, CH ;
Huang, CC ;
Wu, TC ;
Lane, MD ;
Diehl, AM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) :5692-5700
[9]   Induction of UCP2 gene expression by LPS: A potential mechanism for increased thermogenesis during infection [J].
Faggioni, R ;
Shigenaga, J ;
Moser, A ;
Feingold, KR ;
Grunfeld, C .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 244 (01) :75-78
[10]   Uncoupling protein-2: A novel gene linked to obesity and hyperinsulinemia [J].
Fleury, C ;
Neverova, M ;
Collins, S ;
Raimbault, S ;
Champigny, O ;
LeviMeyrueis, C ;
Bouillaud, F ;
Seldin, MF ;
Surwit, RS ;
Ricquier, D ;
Warden, CH .
NATURE GENETICS, 1997, 15 (03) :269-272
1234