Aptamer-Conjugated Multifunctional Nanoflowers as a Platform for Targeting, Capture, and Detection in Laser Desorption Ionization Mass Spectrometry

被引：98

作者：

Ocsoy, Ismail ^{[1
,2
,3
]}

Gulbakan, Basri ^{[1
,2
,3
]}

Shukoor, Mohammed Ibrahim ^{[1
,2
,3
]}

Xiong, Xiangling ^{[1
,2
,3
,4
,5
]}

Chen, Tao ^{[1
,2
,3
]}

Powell, David H. ^{[1
,2
,3
]}

Tan, Weihong ^{[1
,2
,3
,4
,5
]}

机构：

[1] Univ Florida, Ctr Res Bionano Interface, Dept Chem, Gainesville, FL 32611 USA

[2] Univ Florida, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA

[3] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA

[4] Hunan Univ, Mol Sci & Biomed Lab, State Key Lab ChemobioSensing & Chemometr, Coll Biol, Changsha 410082, Hunan, Peoples R China

[5] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

来源：

ACS NANO | 2013年 / 7卷 / 01期

基金：

美国国家卫生研究院;

关键词：

hybrid nanoparticles; nanoflower; aptamer; ATP; cancer cell; mass spectrometry; IRON-OXIDE NANOPARTICLES; GOLD NANOPARTICLES; GRAPHENE OXIDE; MAGNETIC NANOPARTICLES; SELECTIVE ENRICHMENT; SMALL MOLECULES; ION FORMATION; BINDING-SITE; NANO-FLARES; LIVE CELLS;

D O I：

10.1021/nn304458m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Although many different nanomaterials have been tested as substrates for laser desorption and ionization mass spectrometry (LOWS), this emerging field still requires more efficient multifuncional nanomaterials for targeting, enrichment, and detection. Here, we report the use of gold manganese oxide (Au@MnO) hybrid nanoflowers as an efficient matrix for LDI-MS. The nanoflowers were also functionalized with two different aptamers to target cancer cells and capture adenosine triphosphate (ATP). These nanoflowers were successfully used for metabolite extraction from cancer cell lysates. Thus, in one system, OUT multifunctional nanoflowers can (1) act as an ionization substrate for mass spectrometry, (2) target cancer cells, and (3) detect and analyze metabolites from cancer cells.

引用

页码：417 / 427

页数：11

共 78 条

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