Structure and Charge-State Dependence of the Gas-Phase Ionization Energy of Proteins

被引：30

作者：

Giuliani, Alexandre ^{[1
,2
]}

Milosavljevic, Aleksandar R. ^{[5
]}

Hinsen, Konrad ^{[4
]}

Canon, Francis ^{[1
,3
]}

Nicolas, Christophe ^{[1
]}

Refregiers, Matthieu ^{[1
]}

Nahon, Laurent ^{[1
]}

机构：

[1] Synchrotron SOLEIL, F-91192 Gif Sur Yvette, France

[2] INRA, CEPIA, UAR1008, F-44316 Nantes, France

[3] INRA, Ctr Sci Gout & Alimentat, UMR1324, F-21034 Dijon, France

[4] CNRS, Ctr Biophys Mol, UPR4301, F-45071 Orleans, France

[5] Univ Belgrade, Inst Phys, Belgrade 11001, Serbia

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 38期

关键词：

ionization potentials; mass spectrometry; protein structures; X-RAY-SCATTERING; CYTOCHROME-C; IN-VACUO; FOLDING MECHANISMS; MASS-SPECTROMETRY; SPECTROSCOPY; IONS; PRINCIPLES; TRAP;

D O I：

10.1002/anie.201204435

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photoionization of protein ions: The ionization energy of polyprotonated protein cations in the gas phase measured using VUV synchrotron radiation appears to be correlated with the charge state z of the protein and its tertiary structure. A simple electrostatic model accounts for the results and also shows predictive capabilities to derive a mean radius Rm of the protein ion from the ionization energy, and vice versa. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9552 / 9556

页数：5

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