Developments in quantitative mass spectrometry for the analysis of proteome dynamics

被引:20
|
作者
Hughes, Christopher [1 ]
Krijgsveld, Jeroen [1 ]
机构
[1] European Mol Biol Lab, Genome Biol Unit, D-69117 Heidelberg, Germany
关键词
NEWLY SYNTHESIZED PROTEINS; RNA-SEQ; GLOBAL ANALYSIS; MESSENGER-RNA; IN-VIVO; CELLS REVEALS; AMINO-ACIDS; TURNOVER; QUANTIFICATION; SILAC;
D O I
10.1016/j.tibtech.2012.09.007
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two of the primary responses in a cell when perturbed are modulation of the dynamics of its constituent gene expression and protein abundance to restore steady-state homeostasis. To obtain a detailed model of the restoration of this balance, it is necessary to examine the kinetics of transcription and translation, thus going beyond establishing mere abundance levels of transcripts and proteins. In this review we discuss proteomic approaches that utilize genomic tagging and metabolic labeling to reveal turnover kinetics for cellular proteins in a high-throughput manner. Novel metabolic and multiplexed labeling techniques coupled to mass spectrometry, in combination with next-generation sequencing approaches, provide tools for studying the principles of cellular adaptation and dynamics in unprecedented detail.
引用
收藏
页码:668 / 676
页数:9
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