Physicochemical characterization of coumestrol/β-cyclodextrins inclusion complexes by UV-vis and FTIR-ATR spectroscopies

被引：45
作者：
Cannava, C. [2 ]
Crupi, V. [1 ]
Ficarra, P. [2 ]
Guardo, M. [2 ]
Majolino, D. [1 ]
Stancanelli, R. [2 ]
Venuti, V. [1 ]
机构：
[1] Univ Messina, Dipartimento Fis, I-98166 Messina, Italy
[2] Univ Messina, Dipartimento Farmacochim, I-98168 Messina, Italy
来源：
VIBRATIONAL SPECTROSCOPY | 2008年 / 48卷 / 02期
关键词：
UV-vis spectra; FTIR-ATR spectra; Self-deconvolution; Inclusion complex; Coumestrol; beta-Cyclodextrins;
D O I：
10.1016/j.vibspec.2007.12.013
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The inlcusion behaviour of unsubstituted beta-cyclodextrin (beta-CyD) and (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CyD), in solution and solid state was studied with regards to a poorly water-soluble bioflavonoid, coumestrol (Coum), namely 7,12-dihydroxycoumestan, well-known for its anti-oxidant, anti-inflammatory, anti-fungal and anti-viral activities. Phase-solubility measurements were performed to evaluate in solution the complexation of the two cyclodextrins, i.e. beta-CyD and HP-beta-CyD. The stoichiometry and stability constants of the Coum/beta-Cyl) and Coum/HP-beta-CyD complexes were calculated by the phase-solubility method, after which drug-cyclodextrin solid systems were prepared by co-precipitation. In solid phase, the formation of inclusion complexes was confirmed by Fourier transform infrared spectroscopy in attenuated total reflectance (FTIR-ATR) geometry. In particular, complexation mechanisms were explained by the significant differences revealed in the FTIR-ATR spectra of physical mixtures with respect to those of the complexes; the use of deconvolution and curve fitting was determinant. (C) 2008 Elsevier B.V. All rights reserved.
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收藏
页码：172 / 178
页数：7
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