Differential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats

被引：55
作者：
Lucas, Thais F. G. [1 ]
Lazari, Maria Fatima M. [1 ]
Porto, Catarina S. [1 ]
机构：
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 Sao Paulo, Brazil
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2014年 / 382卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
Estrogen receptors; Sertoli cells; CCND1; CDKN1B; GATA-1; DMRT1; NF-KAPPA-B; TRANSCRIPTION FACTOR GATA-1; CYCLIN D1 GENE; FOLLICLE-STIMULATING-HORMONE; ANDROGEN RECEPTOR; FUNCTIONAL MATURATION; TRANSGENIC MOUSE; THYROID-HORMONE; CDK INHIBITORS; MICE LACKING;
D O I：
10.1016/j.mce.2013.09.015
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The aim of the present study was to investigate the role of each estrogen receptors on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats. Activation of ESR1 by 17 beta-estradiol (E2) and ESR1-selective agonist PPT increased CCND1 expression, and this effect was dependent on NF-kB activation. E2 and the ESR2-selective agonist DPN, but not PPT, increased, in a PI3K and CREB-dependent manner, the expression of CDKN1B and the transcription factors GATA-1 and DMRT1. Analyzing the expression of ESR1 and ESR2 in different stages of development of Sefton cells, we observed that the ESR1/ESR2 ratio decreased with age, and this ratio seems to be important to determine the end of cell proliferation and the start of cell differentiation. In Sertoli cells from 15-day-old rats, the ESR1/ESR2 ratio favors the effect of ESR1 and the activation of this receptor increased [Methyl-31-I]thymidine incorporation. We propose that in Sertoli cells from 15-day-old rats E2 modulates Sertoli cell proliferation through ESR1/NF-kappa B-mediated increase of CCND1, and cell cycle exit and differentiation through ESR2/CREB-mediated increase of CDKN1B, GATA-1 and DMRT1. The present study reinforces the important role of estrogen for normal testis development. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：84 / 96
页数：13
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