Production of (S,S)-ethylenediallaine-N,N′ -disuccinic acid from ethylenediamine and fumaric acid by bacteria.

被引:11
作者
Takahashi, R [1 ]
Yamayoshi, K [1 ]
Fujimoto, N [1 ]
Suzuki, M [1 ]
机构
[1] Tokyo Univ Agr, Fac Appl Biosci, Dept Fermentat Sci, Lab Environm Sci Brewing, Tokyo 1568502, Japan
关键词
ethylenediamine-N; N '-disuccinic acid; (S; S)-EDDS; Acidovorax sp; fumaric acid; ethylenediamine;
D O I
10.1271/bbb.63.1269
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We tried to produce (S,S)-ethylenediamine-N,N'-disuccinic acid [(S,S)-EDDS] with the aid of microorganisms that can degrade the compound. A reaction mixture consisting of ethylenediamine and fumaric acid was incubated with the bacteria isolated from soil and sludge, and we found that several kinds of bacteria such as Sphingomonas, Brevundimonas, Pseudomonas, and Acidovorax produced (S,S)-EDDS. The optimum pH and the temperature for the production was pH 7.5 and 35 degrees C for Acidovorax sp. TNT149 and the bacterial cells produced 90 mmoles of (S,S)-EDDS from 200 mmoles of ethylenediamine and 350 mmoles of fumaric acid under these reaction condition.
引用
收藏
页码:1269 / 1273
页数:5
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