Strategies for shotgun identification of integral membrane proteins by tandem mass spectrometry

被引：55
作者：
Lu, Bingwen [1 ]
McClatchy, Daniel B. [1 ]
Kim, Jin Young [1 ]
Yates, John R., III [1 ]
机构：
[1] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA
来源：
PROTEOMICS | 2008年 / 8卷 / 19期
关键词：
Integral membrane protein; Membrane protein enrichment; Sample preparation; Transmembrane domain;
D O I：
10.1002/pmic.200800120
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Integral membrane proteins (IMPs) are difficult to identify, mainly for two reasons: the hydrophobicity of IMPs and their low abundance. Sample preparation is a key component in the large-scale identification of IMPs. In this review, we survey strategies for shotgun identification of IMPs by MS/MS. We will discuss enrichment, solubilization, separation, and digestion of IMPs, and data analysis for membrane proteomics.
引用
收藏
页码：3947 / 3955
页数：9
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