Arabinose 5-phosphate analogues as mechanistic probes for Neisseria meningitidis 3-deoxy-D-manno-octulosonate 8-phosphate synthase

被引：9

作者：

Ahn, Meekyung ^{[2
]}

Cochrane, Fiona C. ^{[2
]}

Patchett, Mark L. ^{[3
]}

Parker, Emily J. ^{[1
]}

机构：

[1] Univ Canterbury, Dept Chem, Christchurch 1, New Zealand

[2] Massey Univ, Inst Fundamental Sci, Palmerston North, New Zealand

[3] Massey Univ, Inst Mol Biosci, Palmerston North, New Zealand

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 22期

关键词：

Neisseria meningitidis; D-A5P; KDOP; Lipopolysaccharide biosynthesis;

D O I：

10.1016/j.bmc.2008.09.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3-Deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase catalyses the condensation reaction between phosphoenolpyruvate and D-arabinose 5-phosphate (D-A5P) in a key step in lipopolysaccharide biosynthesis in Gram-negative bacteria. The KDO8P synthase from Neisseria meningitidis was cloned into Escherichia coli, overexpressed and purified. A variety of D-A5P stereoisomers were tested as substrates, of these only D-A5P and L-X5P were substrates. The Asn59Ala mutant of N. meningitidis KDO8P synthase was constructed and this mutant retained less than 1% of the wild-type activity. These results are consistent with a catalytic mechanism for this enzyme in which the C2 and C3 hydroxyl groups of D-A5P and Asn59 are critical. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：9830 / 9836

页数：7

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