E-coli metabolic engineering for gram scale production of a plant-based anti-inflammatory agent

被引:34
作者
Ahmadi, Mahmoud Kamal [1 ]
Fang, Lei [1 ]
Moscatello, Nicholas [1 ]
Pfeifer, Blaine A. [1 ]
机构
[1] Univ Buffalo State Univ New York, Dept Chem & Biol Engn, Buffalo, NY 14260 USA
基金
美国国家科学基金会;
关键词
Salicylate; 2-O-beta-D-glucoside; Plant; E; coli; Anti-inflammatory; Co-culture; SALICYLIC-ACID GLUCOSYLTRANSFERASE; UDP-GLUCOSE; POLYKETIDE; BIOSYNTHESIS; PURIFICATION; EXPRESSION; PRECURSOR; PATHWAY; IRP9;
D O I
10.1016/j.ymben.2016.10.001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this report, the heterologous production of salicylate (SA) is the basis for metabolic extension to salicylate 2-O-beta-D-glucoside (SAG), a natural product implicated in plant-based defense mechanisms. Production was optimized through a combination of metabolic engineering, gene expression variation, and co-culture design. When combined, SA and SAG production titers reached -0.9 g/L and -2.5 g/L, respectively. The SAG compound was then tested for anti-inflammatory properties relative to SA and acetylsalicylate (aspirin). Results indicate comparable activity between SAG and aspirin in reducing nitric oxide (NO) and reactive oxygen species (ROS) from macrophage cells while no discernable negative effects on cellular viability were observed.
引用
收藏
页码:382 / 388
页数:7
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