Lipase-catalyzed synthesis of ferulyl oleins in solvent-free medium

被引:26
作者
Xin, Jia-ying [1 ,2 ]
Zhang, Lei
Chen, Lin-lin
Zheng, Yan
Wu, Xiao-mei [2 ]
Xia, Chun-gu [2 ]
机构
[1] Harbin Univ Commerce, Coll Food Engn, Key Lab Food Sci & Engn, Harbin 150076, Heilongjiang, Peoples R China
[2] Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China
关键词
Ethyl ferulate; Ferulyl diolein; Ferulyl monoolein; Glycerol; Lipase; Triolein; Vacuum-rotary evaporation procedure; FERULOYLATED MONOACYL-GLYCEROLS; PHENOLIC-ACIDS; ENZYMATIC-SYNTHESIS; CANDIDA-ANTARCTICA; ALKYL FERULATE; DIACYLGLYCEROLS; DERIVATIVES; ANTIOXIDANTS; TRIOLEIN; OILS;
D O I
10.1016/j.foodchem.2008.06.024
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Lipase-catalyzed transesterification of ethyl ferulate (EF) with triolein (TO), to form ferulyl oleins in a solvent-free medium, was investigated. Water activity (a(w)) had an obvious influence on transesterification efficacy. A possible explanation is that the reaction of EF with TO would be deacylation-rate determined. In order to enhance the combined yield of ferulyl diolein (FDO) and ferulyl monoolein (FMO) and minimize hydrolysis of substrate, glycerol (rather than water) was introduced to the reaction. A maximal combined yield of 29.0% of FDO and FMO was obtained using a 1:3 mole ratio of glycerol to TO in solvent-free medium at 60 degrees C. Compared to the ferulyl olein synthesis conducted by the normal pressure magnetic stirring procedure, the synthesis of ferulyl oleins by a vacuum-rotary evaporation procedure, with a vacuum of 7.5 mmHg, affords higher yields within the same time. The enzyme can be reused several times without significant loss of activity. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:640 / 645
页数:6
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