Comparison of the influence of Cu, Zn, and Cd on the activity and kinetics of free and immobilized acid phosphatase

被引：0

作者：

Huang, QY ^{[1
]}

Shindo, H

机构：

[1] Yamaguchi Univ, Fac Agr, Yamaguchi 7538515, Japan

[2] Huazhong Agr Univ, Dept Resources Environm & Agrochem, Wuhan 430070, Peoples R China

来源：

SOIL SCIENCE AND PLANT NUTRITION | 2001年 / 47卷 / 04期

关键词：

acid phosphatase; heavy metal; immobilized enzyme; mineral; soil clay;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The influence of Cu, Zn, and Cd on the activity and kinetics of acid phosphatase immobilized by two soil clays, kaolin or goethite was investigated. The ability of Cu to inhibit the enzyme activity was higher than that of Zn in all the enzyme complexes examined. The ability of Cd was negligible. The inhibitory effects of Cu and Zn on the two soil clay- and kaolin-enzyme complexes were much stronger than those on the goethite-enzyme complex. The V-max and K-m values of the enzyme complexes indicated that both Cu and Zn decreased the maximum reaction velocity of the enzymes, but increased the affinity of the enzymes for the substrate. The degree of the decrease and increase was higher in the Cu systems than in the Zn systems.

引用

页码：767 / 772

页数：6

共 17 条

[1] ADSORPTION OF POTENTIAL-DETERMINING IONS AT FERRIC OXIDE-AQUEOUS ELECTROLYTE INTERFACE [J].