EVALUATION OF A SIMPLE THEILERIA ANNULATA CULTURE PROTOCOL FROM EXPERIMENTALLY INFECTED BOVINE WHOLE BLOOD

被引：4

作者：

Gharbi, M. ^{[1
]}

Latrach, R. ^{[1
]}

Sassi, L. ^{[1
]}

Darghouth, M. A. ^{[1
]}

机构：

[1] Univ Manouba, Ecole Natl Med Vet, Parasitol Lab, Sidi Thabet 2020, Tunisia

来源：

PARASITE | 2012年 / 19卷 / 03期

关键词：

Theileria annulata; cell culture; blood; Ficoll;

D O I：

10.1051/parasite/2012193281

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We have evaluated a new simple technique using whole blood from experimentally infected cattle for the isolation and cultivation of Theileria annulata. The study was carried out on 20 Holstein-Frisian bovines that had been experimentally infected with a virulent lethal dose of Theileria annulata. This technique has been compared to he classical peripheral blood monocyte isolation with Ficoll caned out on 22 experimentally infected Holstein-Friesian calves. The effectiveness of the reference technique was estimated to 86.4 %, whilst the effectiveness of the new technique was 100 %. Moreover, this new technique leads to time and money saving estimated to (sic) 3.06 per sample. It decreases the contamination risks by reducing the steps of sample manipulation.

引用

页码：281 / 283

页数：3

共 13 条

[1]

Brown C G, 1990, Parassitologia, V32, P23

[2]

Brown C. G. D., 1981, Advances in the Control of Theileriosis. Proceedings of an International Conference held at the International Laboratory for Research on Animal Diseases in Nairobi, 9-13th February, 1981, P104

[3]

Darghouth M. A., 2003, MALADIES INFECT PARA, P1585

[4] A preliminary study on the attenuation of Tunisian schizont-infected cell lines of Theileria annulata [J].

Darghouth, MA ;

BenMiled, L ;

Bouattour, A ;

Melrose, TR ;

Brown, CGD ;

Kilani, M .

PARASITOLOGY RESEARCH, 1996, 82 (07) :647-655

[5]

DARGHOUTH MA, 2000, THESIS I NATL POLYTE

[6] EXPRESSION OF TAC ANTIGEN COMPONENT OF BOVINE INTERLEUKIN-2 RECEPTOR IN DIFFERENT LEUKOCYTE POPULATIONS INFECTED WITH THEILERIA-PARVA OR THEILERIA-ANNULATA [J].

DOBBELAERE, DAE ;

PROSPERO, TD ;

RODITI, IJ ;

KELKE, C ;

BAUMANN, I ;

EICHHORN, M ;

WILLIAMS, RO ;

AHMED, JS ;

BALDWIN, CL ;

CLEVERS, H ;

MORRISON, WI .

INFECTION AND IMMUNITY, 1990, 58 (12) :3847-3855

[7] Infection of calves with Theileria annulata in Tunisia:: Economic analysis and evaluation of the potential benefit of vaccination [J].

Gharbi, M ;

Sassi, L ;

Dorchies, P ;

Darghouth, MA .

VETERINARY PARASITOLOGY, 2006, 137 (3-4) :231-241

[8]

GHARBI M., 2006, THESIS I NATL POLYTE

[9] The balance between protective immunity and pathogenesis in tropical theileriosis: what we need to know to design effective vaccines for the future [J].

Glass, EJ .

RESEARCH IN VETERINARY SCIENCE, 2001, 70 (01) :71-75

[10]

Pipano E, 1990, Rev Elev Med Vet Pays Trop, V42, P529

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