Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador

被引:31
作者
Garcia-Tenesaca, Marilyn [1 ]
Navarrete, Eillen S. [1 ]
Iturralde, Gabriel A. [2 ]
Villacres Granda, Irina M. [2 ]
Tejera, Eduardo [3 ]
Beltran-Ayala, Pablo [4 ]
Giampieri, Francesca [5 ]
Battino, Maurizio [5 ]
Alvarez-Suarez, Jose M. [6 ]
机构
[1] Univ Las Amer, Fac Ingn & Ciencias Agr, Quito 170125, Ecuador
[2] Univ Las Amer, Labs Invest, Quito 170125, Ecuador
[3] Univ Las Amer, Grp Bioquimioinformat, Fac Ingn & Ciencias Agr, Quito 170125, Ecuador
[4] Univ San Francisco Quito, Colegio Adm & Econ, Cumbaya 170157, Quito, Ecuador
[5] Univ Politecn Marche, Fac Med, Sez Biochim, Dipartimento Sci Clin Specialist & Odontostomatol, I-60121 Ancona, Italy
[6] Univ Las Amer, Escuela Med Vet & Zootecnia, Grp Invest Biotecnol Aplicada Biomed BIOMED, Quito 170125, Ecuador
关键词
Ecuadorian monofloral honey; avocado honey; Eucalyptus honey; Rapeseed honey; ANTIOXIDANT CAPACITY; COLOR;
D O I
10.3390/ijms19010045
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three types of monofloral honey from the Andean regions of Ecuador (Avocado, Eucalyptus, and Rapeseed honey) were analyzed to determine their floral origin, physicochemical parameters, chemical composition, antioxidant capacity, and their capacity to reduce in vitro bacterial biofilms. The chemical composition varied considerably depending on floral origin. The highest values of bioactive compounds were found in Avocado honey, classified as dark amber in color, while the lowest values were found in Eucalyptus honey followed by Rapeseed honey, both classified as extra light amber. When compared to Eucalyptus and Rapeseed honey, Avocado honey showed a more effective superoxide scavenging activity, chelating metal ions capacity, and a higher ability to protect human erythrocyte membranes against lipid peroxidation. For antimicrobial activity, the hydrogen peroxide content and the capacity to inhibit the biofilm formation, and to remove preformed biofilm from Staphylococcus aureus and Klebsiella pneumoniae was determined. Avocado honey showed the highest values of hydrogen peroxide content, as well as the highest capacity to reduce in vitro bacterial biofilms. A correlation between color vs. phenolics content vs. superoxide scavenging activity vs. chelating metal ions capacity, and the capacity to protect human erythrocyte membranes against lipid peroxidation was found.
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页数:15
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