Glycomic approach for potential biomarkers on prostate cancer: Profiling of N-linked glycans in human sera and pRNS cell lines

被引：71

作者：

de Leoz, Maria Lorna A. ^{[1
]}

An, Hyun Joo ^{[1
]}

Kronewitter, Scott ^{[1
]}

Kim, Jaehan ^{[2
]}

Beecroft, Sean ^{[1
]}

Vinall, Ruth ^{[3
]}

Miyamoto, Suzanne ^{[4
]}

White, Ralph de Vere ^{[4
]}

Lam, Kit S. ^{[4
]}

Lebrilla, Carlito ^{[1
,5
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA

[3] Univ Calif Davis, Med Ctr, Dept Urol, Sacramento, CA 95817 USA

[4] Univ Calif Davis, Med Ctr, Div Hematol & Oncol, Sacramento, CA 95817 USA

[5] Univ Calif Davis, Sch Med, Dept Biochem, Davis, CA 95616 USA

来源：

DISEASE MARKERS | 2008年 / 25卷 / 4-5期

关键词：

Biomarker; glycans; mass spectrometry; prostate cancer; serum;

D O I：

10.1155/2008/515318

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.

引用

页码：243 / 258

页数：16

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