Novel impedimetric dopamine biosensor based on boronic acid functional polythiophene modified electrodes

In this study we report a new, simple and first impedimetric biosensor based on 3-Thienyl boronic acid for dopamine detection. Biosensor electrode preparation is 1 min long by simple electro-polymerization of 3-Thienyl boronic acid and copolymer Thiophene P(TBA(0.50)Th(0.50)). Strong interaction between dopamine and thin layer of boronic acid has provided bio-sensing electrode high selectivity and stability, linear range of 7.8 to 125 mu M, and detection limit of 0.3 mu M. Characterization and optimization studies were conducted using electrochemical impedance spectroscopy (EIS) and cyclic voltammogram (CV). In order to test reliability of proposed biosensor real sample application study has been conducted using non-diluted human urine and it has been found that biosensor selectivity and recovery is excellent. As well P(TBA(0.50)Th(0.50)) based electrode and dopamine interaction has been proven by single frequency impedance measurements. Biosensors acquired good reproducibility, stability, selectivity and very low interference. (C) 2016 Elsevier B.V. All rights reserved.