The effect of mesenchymal stem cells on osteoclast precursor cell differentiation

被引：19

作者：

Abe, Takaharu ^{[1
]}

Sumi, Keisuke ^{[1
]}

Kunimatsu, Ryo ^{[1
]}

Oki, Nanae ^{[1
]}

Tsuka, Yuji ^{[1
]}

Nakajima, Kengo ^{[2
]}

Ando, Kazuyo ^{[2
]}

Tanimoto, Kotaro ^{[1
]}

机构：

[1] Hiroshima Univ Hosp, Div Oral Hlth & Dev, Dept Orthodont, Hiroshima, Japan

[2] Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Orthodont Appl Life Sci, Hiroshima, Japan

来源：

JOURNAL OF ORAL SCIENCE | 2019年 / 61卷 / 01期

关键词：

mesenchymal stem cell; osteoclast precursor cell; differentiation; bone regeneration; OSTEOBLASTIC CELLS; CLEFT-LIP; EXPRESSION; SUPPRESSION; MONOCYTES; ALVEOLAR; PART;

D O I：

10.2334/josnusd.17-0315

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Transplantation of mesenchymal stem cells (MSCs) has been extensively studied in the field of regenerative medicine. Bone regeneration is achieved via the interaction of osteoblasts and osteoclasts. However, the influence of MSCs on osteoclasts is unknown. The purpose of this study was to investigate the effect of MSCs on the expression of genes for osteoclast differentiation factors using qPCR after indirect co-culture of MSCs and RAW264 cells. The numbers of osteoclasts after addition of soluble receptor activator of nuclear factor kappa B (NF-kappa B) ligand (sRANKL) were also compared. Expression of osteoprotegerin (OPG) by MSCs was significantly elevated in co-culture over time. The differentiation of RAW264 cells into mature osteoclasts following addition of sRANKL was significantly inhibited by co-culture with MSCs. Expression of RANK, colony stimulating factor 1 receptor, NF-kappa B, and nuclear factor of activated T-cell cytoplasmic 1 in RAW264 cells was significantly inhibited by co-culture with MSCs. Expression of OPG protein was higher in co-culture with RAW264 cells than in MSCs alone, and the expression level was clearly higher than that of RANKL. MSCs appeared to inhibit osteoclast differentiation via expression of OPG.

引用

页码：30 / 35

页数：6

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