Effects of chronic dietary selenomethionine exposure on repeat swimming performance, aerobic metabolism and methionine catabolism in adult zebrafish (Danio rerio)

被引:53
作者
Thomas, Jith K. [1 ]
Wiseman, Steve [1 ]
Giesy, John P. [1 ,2 ,3 ,4 ,5 ,6 ]
Janz, David M. [1 ,2 ]
机构
[1] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
[2] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B4, Canada
[3] Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA
[4] Michigan State Univ, Ctr Integrat Toxicol, E Lansing, MI 48824 USA
[5] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
[6] City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Hong Kong, Peoples R China
基金
加拿大自然科学与工程研究理事会;
关键词
Selenium; U-crit; Energetics; Swim performance; Metabolic rate; Aerobic scope; Zebrafish; TROUT ONCORHYNCHUS-MYKISS; TYROSINE-PHOSPHATASE; 1B; S-ADENOSYLMETHIONINE; ENERGY HOMEOSTASIS; HEPATIC STEATOSIS; SELENIUM; TOXICITY; LIVER; EXERCISE; MUSCLE;
D O I
10.1016/j.aquatox.2013.01.009
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
In a previous study we reported impaired swimming performance and greater stored energy in adult zebrafish (Danio rerio) after chronic dietary exposure to selenomethionine (SeMet). The goal of the present study was to further investigate effects of chronic exposure to dietary SeMet on repeat swimming performance, oxygen consumption (MO2), metabolic capacities (standard metabolic rate [SMR]; active metabolic rate [AMR], factorial aerobic scope [F-AS] and cost of transport [COT]) and gene expression of energy metabolism and methionine catabolism enzymes in adult zebrafish. Fish were fed SeMet at measured concentrations of 1.3, 3.4, 9.8 or 27.5 mu g Se/g dry mass (d.m.) for 90 d. At the end of the exposure period, fish from each treatment group were divided into three subgroups: (a) no swim, (b) swim, and (c) repeat swim. Fish from the no swim group were euthanized immediately at 90 d and whole body tri-glycerides, glycogen and lactate, and gene expression of energy metabolism and methionine catabolism enzymes were determined. Individual fish from the swim group were placed in a swim tunnel respirometer and swimming performance was assessed by determining the critical swimming speed (U-crit). After both U-crit and MO2 analyses, fish were euthanized and whole body energy stores and lactate were determined. Similarly, individual fish from the repeat swim group were subjected to two U-crit tests (Ucrit-1 and Ucrit-2) performed with a 60 min recovery period between tests, followed by determination of energy stores and lactate. Impaired swim performance was observed in fish fed SeMet at concentrations greater than 3 mu g Se/g in the diet. However, within each dietary Se treatment group, no significant differences between single and repeat U-crits were observed. Oxygen consumption, SMR and COT were significantly greater, and F-AS was significantly lesser, in fish fed SeMet. Whole body triglycerides were proportional to the concentration of SeMet in the diet. While swimming resulted in lesser concentrations of glycogen in the body, exposure to SeMet in the diet had no significant effect on glycogen content. Exposure to SeMet significantly down-regulated mRNA abundance of protein tyrosine phosphatase 1B (PTP 1B) in muscle, and beta-hydroxyacyl coenzyme A dehydrogenase (HOAD), sterol regulatory element binding protein 1 (SREBP 1) and methionine adenosyltransferase 1 alpha (MAT 1A) in liver of adult zebrafish. Overall the results of this study suggest chronic exposure of adult zebrafish to SeMet in the diet can cause both cellular and organismal effects that could affect fitness and survivability of fish. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:112 / 122
页数:11
相关论文
共 66 条
[41]   Selenium and diabetes: an enigma? [J].
Mueller, Andreas S. ;
Mueller, Kristin ;
Wolf, Nicole M. ;
Pallauf, Josef .
FREE RADICAL RESEARCH, 2009, 43 (11) :1029-1059
[42]   Regulation of the insulin antagonistic protein tyrosine phosphatase 1B by dietary Se studied in growing rats [J].
Mueller, Andreas S. ;
Bosse, Astrid C. ;
Most, Erika ;
Klomann, Sandra D. ;
Schneider, Sandra ;
Pallauf, Josef .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2009, 20 (04) :235-247
[43]   Oxidative stress and muscle homeostasis [J].
Musaro, Antonio ;
Fulle, Stefania ;
Fano, Giorgio .
CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, 2010, 13 (03) :236-242
[44]   Metabolism of selenomethionine by rainbow trout (Oncorhynchus mykiss) embryos can generate oxidative stress [J].
Palace, VP ;
Spallholz, JE ;
Holm, J ;
Wautier, K ;
Evans, RE ;
Baron, CL .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2004, 58 (01) :17-21
[45]   The physiological consequences of exposure to chronic, sublethal waterborne nickel in rainbow trout (Oncorhynchus mykiss):: exercise vs resting physiology [J].
Pane, EF ;
Haque, A ;
Goss, GG ;
Wood, CM .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2004, 207 (07) :1249-1261
[46]   Selenium uptake and speciation in wild and caged fish downstream of a metal mining and milling discharge [J].
Phibbs, James ;
Wiramanaden, Cheryl I. E. ;
Hauck, Dominic ;
Pickering, Ingrid J. ;
Liber, Karsten ;
Janz, David M. .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2011, 74 (05) :1139-1150
[47]   Critical swimming speed: its ecological relevance [J].
Plaut, I .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2001, 131 (01) :41-50
[48]  
Priede I.G., 1985, P33
[49]   Effects of environmental metal contamination on the condition, swimming performance, and tissue metabolic capacities of wild yellow perch (Perca flavescens) [J].
Rajotte, JW ;
Couture, P .
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 2002, 59 (08) :1296-1304
[50]   Mechanisms of hepatic steatosis in mice fed a lipogenic methionine choline-deficient diet [J].
Rinella, Mary E. ;
Elias, Marc S. ;
Smolak, Robin R. ;
Fu, Tao ;
Borensztajn, Jayme ;
Green, Richard M. .
JOURNAL OF LIPID RESEARCH, 2008, 49 (05) :1068-1076