Development of a chemical probe for identifying protein targets of α-oxoaldehydes

被引：34
作者：
Sibbersen, Christian [1 ,2 ]
Palmfeldt, Johan [3 ]
Hansen, Jakob [2 ]
Gregersen, Niels [3 ]
Jorgensen, Karl Anker [1 ]
Johannsen, Mogens [2 ]
机构：
[1] Aarhus Univ, Dept Chem, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ, Bioanalyt Unit, Dept Forens Med, DK-8200 Aarhus N, Denmark
[3] Aarhus Univ Hosp, Inst Clin Med, Res Unit Mol Med, DK-8200 Aarhus N, Denmark
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 38期
关键词：
HUMAN SERUM-ALBUMIN; DIABETIC COMPLICATIONS; ENDOTHELIAL-CELLS; TERMINAL ALKYNES; METHYLGLYOXAL; GLYCATION; METABOLISM; INCREASES; BINDING; AZIDES;
D O I：
10.1039/c3cc41099d
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The development of a chemical probe for identifying the protein targets of reactive electrophilic alpha-oxoaldehydes such as methylglyoxal is presented. The probe is evaluated against methylglyoxal using human serum albumin as well as using living cells and lysates.
引用
收藏
页码：4012 / 4014
页数：3
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共 33 条
  • [1] Protein modification and replicative senescence of WI-38 human embryonic fibroblasts
    Ahmed, Emad K.
    Rogowska-Wrzesinska, Adelina
    Roepstorff, Peter
    Bulteau, Anne-Laure
    Friguet, Bertrand
    [J]. AGING CELL, 2010, 9 (02) : 252 - 272
  • [2] Chromatographic assay of glycation adducts in human serum albumin glycated in vitro by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and intrinsic fluorescence
    Ahmed, N
    Thornalley, PJ
    [J]. BIOCHEMICAL JOURNAL, 2002, 364 (01) : 15 - 24
  • [3] Peptide mapping identifies hotspot site of modification in human serum albumin by methylglyoxal involved in ligand binding and esterase activity
    Ahmed, N
    Dobler, D
    Dean, M
    Thornalley, PJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (07) : 5724 - 5732
  • [4] The Chaperone-Dependent Ubiquitin Ligase CHIP Targets HIF-1α for Degradation in the Presence of Methylglyoxal
    Bento, Carla Figueira
    Fernandes, Rosa
    Ramalho, Jose
    Marques, Carla
    Shang, Fu
    Taylor, Allen
    Pereira, Paulo
    [J]. PLOS ONE, 2010, 5 (11):
  • [5] Seeing Citrulline: Development of a Phenylglyoxal-Based Probe To Visualize Protein Citrullination
    Bicker, Kevin L.
    Subramanian, Venkataraman
    Chumanevich, Alexander A.
    Hofseth, Lorne J.
    Thompson, Paul R.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (41) : 17015 - 17018
  • [6] Methylglyoxal modification of Nav1.8 facilitates nociceptive neuron firing and causes hyperalgesia in diabetic neuropathy
    Bierhaus, Angelika
    Fleming, Thomas
    Stoyanov, Stoyan
    Leffler, Andreas
    Babes, Alexandru
    Neacsu, Cristian
    Sauer, Susanne K.
    Eberhardt, Mirjam
    Schnoelzer, Martina
    Lasischka, Felix
    Neuhuber, Winfried L.
    Kichko, Tatjana I.
    Konrade, Ilze
    Elvert, Ralf
    Mier, Walter
    Pirags, Valdis
    Lukic, Ivan K.
    Morcos, Michael
    Dehmer, Thomas
    Rabbani, Naila
    Thornalley, Paul J.
    Edelstein, Diane
    Nau, Carla
    Forbes, Josephine
    Humpert, Per M.
    Schwaninger, Markus
    Ziegler, Dan
    Stern, David M.
    Cooper, Mark E.
    Haberkorn, Uwe
    Brownlee, Michael
    Reeh, Peter W.
    Nawroth, Peter P.
    [J]. NATURE MEDICINE, 2012, 18 (06) : 926 - +
  • [7] Fast and efficient MCR-based synthesis of clickable rhodamine tags for protein profiling
    Brauch, Sebastian
    Henze, Michael
    Osswald, Bianca
    Naumann, Kai
    Wessjohann, Ludger A.
    van Berkel, Sander S.
    Westermann, Bernhard
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2012, 10 (05) : 958 - 965
  • [8] Biochemistry and molecular cell biology of diabetic complications
    Brownlee, M
    [J]. NATURE, 2001, 414 (6865) : 813 - 820
  • [9] Methylglyoxal, protein binding and biological samples: Are we getting the true measure?
    Dhar, Arti
    Desai, Kaushik
    Liu, Jianghai
    Wu, Lingyun
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (11-12): : 1093 - 1100
  • [10] Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal
    Distler, Margaret G.
    Plant, Leigh D.
    Sokoloff, Greta
    Hawk, Andrew J.
    Aneas, Ivy
    Wuenschell, Gerald E.
    Termini, John
    Meredith, Stephen C.
    Nobrega, Marcelo A.
    Palmer, Abraham A.
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2012, 122 (06) : 2306 - 2315
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