Effect of L-arginine on HSP70 expression in liver in weanling piglets

被引：29

作者：

Wu, Xin ^{[1
,2
]}

Xie, Chunyan ^{[1
]}

Yin, Yulong ^{[1
,2
,3
]}

Li, Fengna ^{[1
]}

Li, Tiejun ^{[1
]}

Huang, Ruilin ^{[1
]}

Ruan, Zheng ^{[2
]}

Deng, Zeyuan ^{[2
]}

机构：

[1] Chinese Acad Sci, Inst Subtrop Agr, Hunan Prov Engn Res Ctr Hlth Livestock,Key Lab Ag, Minist Agr,Sci Observing & Expt Stn Anim Nutr & F, Changsha 410125, Hunan, Peoples R China

[2] Nanchang Univ, State Key Lab Food Sci & Technol & Coll, Nanchang 330031, Peoples R China

[3] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China

来源：

BMC VETERINARY RESEARCH | 2013年 / 9卷

基金：

中国博士后科学基金;

关键词：

L-arginine; Liver; HSP70; Weanling piglets; SMALL-INTESTINAL STRUCTURE; HEAT-SHOCK; HEAT-SHOCK-PROTEIN-70; SUPPLEMENTATION; STRESS; PROTEINS; DEFICIENCY; NUTRITION; GROWTH; SERUM;

D O I：

10.1186/1746-6148-9-63

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: This study was conducted to evaluate the effects of L-arginine (Arg) on photomicrographs and HSP70 expression in the liver of weanling piglets. Twelve healthy Landrace x Yorkshire piglets that had been weaned at 21 d (average body weight 5.56 +/- 0.51 kg) were randomly divided into a control group and an Arg group (6 g/kg feed). At age 28 d, all of the piglets were slaughtered to obtain liver samples to determine HSP70 expression by real-time PCR, western blot and immunohistochemistry. Results: The results showed that, compared to control piglets, treatment with Arg decreased inflammatory reactions caused by weaning. The immunohistochemical localization of HSP70 in liver revealed strong expression in the Arg group. Arg increased HSP70 mRNA and HSP70 expression in the liver (P < 0.05). Conclusions: These findings suggest that dietary supplementation with Arg could maintain liver health by inducing HSP70 expression in weanling piglets.

引用

页数：6

共 34 条

[1]

[Anonymous], 1998, NUTR REQ SWIN

[2] INTERACTION OF HSP-70 WITH NEWLY SYNTHESIZED PROTEINS - IMPLICATIONS FOR PROTEIN FOLDING AND ASSEMBLY [J].

BECKMANN, RP ;

MIZZEN, LA ;

WELCH, WJ .

SCIENCE, 1990, 248 (4957) :850-854

[3] Heat-shock protein 70 favours human liver recovery from ischaemia-reperfusion [J].

Boeri, D ;

Dondero, F ;

Storace, D ;

Maiello, M ;

Pasqualini, M ;

Pellicci, R .

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2003, 33 (06) :500-504

[4] Weaning induces both transient and long-lasting modifications of absorptive, secretory, and barrier properties of piglet intestine [J].

Boudry, G ;

Péron, V ;

Le Huërou-Luron, I ;

Lallès, JP ;

Sève, B .

JOURNAL OF NUTRITION, 2004, 134 (09) :2256-2262

[5]

Calabrese F, 1997, J PATHOL, V183, P477

[6] Changes in caecal microbiota and mucosal morphology of weaned pigs [J].

Castillo, Marisol ;

Martin-Orue, Susana M. ;

Nofrarias, Miquel ;

Manzanilla, Edgar G. ;

Gasa, Josep .

VETERINARY MICROBIOLOGY, 2007, 124 (3-4) :239-247

[7] Therapeutic treatment with L-arginine rescues mice from heat stroke-induced death: Physiological and molecular mechanisms [J].

Chatterjee, S ;

Premachandran, S ;

Sharma, D ;

Bagewadikar, RS ;

Poduval, TB .

SHOCK, 2005, 24 (04) :341-347

[8] Role of nitric oxide in liver injury [J].

Chen, T ;

Zamora, R ;

Zuckerbraun, B ;

Billiar, TR .

CURRENT MOLECULAR MEDICINE, 2003, 3 (06) :519-526

[9] Dossier: Free amino acids in human health and pathologies - The metabolic basis of arginine nutrition and pharmacotherapy [J].

Flynn, NE ;

Meininger, CJ ;

Haynes, TE ;

Wu, G .

BIOMEDICINE & PHARMACOTHERAPY, 2002, 56 (09) :427-438

[10] Induction, modification and accumulation of HSP70s in the rat liver after acute exercise:: early and late responses [J].

González, B ;

Manso, R .

JOURNAL OF PHYSIOLOGY-LONDON, 2004, 556 (02) :369-385

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