Morphological and biochemical analysis of Rac1 in three-dimensional epithelial cell cultures

被引：43

作者：

O'Brien, LE ^{[1
]}

Yu, W ^{[1
]}

Tang, K ^{[1
]}

Jou, TS ^{[1
]}

Zegers, MMP ^{[1
]}

Mostov, KE ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY | 2006年 / 406卷

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0076-6879(06)06053-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rho GTPases are critical regulators of epithelial morphogenesis. A powerful means to investigate their function is three-dimensional (3D) cell culture, which mimics the architecture of epithelia in vivo. However, the nature of 3D culture requires specialized techniques for morphological and biochemical analyses. Here, we describe protocols for 3D culture studies with Madin-Darby Canine Kidney (MDCK) epithelial cells: establishing cultures, immunostaining, and expressing, detecting, and assaying Rho proteins. These protocols enable the regulation of epithelial morphogenesis to be explored at a detailed molecular level.

引用

页码：676 / 691

页数：16

共 50 条

[41] EFFECTS OF HYPOXIA ON MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF RENAL EPITHELIAL-CELL AND TUBULE CULTURES
ALLEN, J
WINTERFORD, C
AXELSEN, RA
GOBE, GC
RENAL FAILURE, 1992, 14 (04) : 453 - 460
[42] Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures
Bougault, Carole
Paumier, Anne
Aubert-Foucher, Elisabeth
Mallein-Gerin, Frederic
NATURE PROTOCOLS, 2009, 4 (06) : 928 - 938
[43] Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures
Carole Bougault
Anne Paumier
Elisabeth Aubert-Foucher
Frédéric Mallein-Gerin
Nature Protocols, 2009, 4 : 928 - 938
[44] Purification and biochemical properties of Rac1, 2, 3 and the splice variant Rac1b
Haeusler, LC
Hemsath, L
Fiegen, D
Blumenstein, L
Herbrand, U
Stege, P
Dvorsky, R
Ahmadian, MR
METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY, 2006, 406 : 1 - 11
[45] Three-Dimensional Analysis of Corneal Epithelial Nerves in Diabetes
Yorek, Matthew Scott
Yorek, Mark A.
Kardon, Randy H.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2014, 55 (13)
[46] Role of Rac1 in Alveolar Epithelial Cells in Apoptotic Cell Removal During Lung Development
Kadl, A.
Desmett, A.
Jayaguru, P.
Tundup, S.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2018, 197
[47] Correction: Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures
Michael Killinger
Adéla Kratochvilová
Eva Ingeborg Reihs
Eva Matalová
Karel Klepárník
Mario Rothbauer
Journal of Biological Engineering, 19 (1)
[48] FIB Preparation and SEM Investigations for Three-Dimensional Analysis of Cell Cultures on Microneedle Arrays
Friedmann, A.
Cismak, A.
Tautorat, C.
Koester, P. J.
Baumann, W.
Held, J.
Gaspar, J.
Ruther, P.
Paul, O.
Heilmann, A.
SCANNING, 2012, 34 (04) : 221 - 229
[49] The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures
Blondeel, Eva
Peirsman, Arne
Vermeulen, Stephanie
Piccinini, Filippo
De Vuyst, Felix
Estevao, Diogo
Al-Jamei, Sayida
Bedeschi, Martina
Castellani, Gastone
Cruz, Tania
Dedeyne, Sandor
Oliveira, Maria Jose
Kawakita, Satoru
Nguyen, Huu Tuan
Kunz-Schughart, Leoni A.
Lee, Soojung
Marino, Noemi
Steigemann, Patrick
Takayama, Shuichi
Tesei, Anna
Zablowsky, Nina
Blondeel, Phillip
De Wever, Olivier
SCIENTIFIC DATA, 2025, 12 (01)
[50] Rac1 GTPases are Activated during Galectin-3-Induced Epithelial Cell Scattering
Saravanan, Chandrassegar
Liu, Fu-Tong
Panjwani, Noorjahan
GLYCOBIOLOGY, 2008, 18 (11) : 988 - 988

← 1 2 3 4 5 →