A fluorometric determination of urea with urease

被引：6

作者：

Suye, S

Miura, J

Ohue, M

机构：

[1] Fukui Univ, Dept Appl Chem & Biotechnol, Fukui 910, Japan

[2] Fukui Univ, Ctr Instrumental Anal, Fukui 910, Japan

[3] Nara Natl Coll Technol, Dept Chem Engn, Nara 63911, Japan

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 08期

关键词：

urease; fluorometric method; urea; sodium fluorescein;

D O I：

10.1080/00032719908542914

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive fluorometric method was developed for the determination of urea. The method was based on the hydrolyzation of urea catalyzed by urease with pH and the change of fluorescence intensity of sodium fluorescein as a pH sensitive dye in the enzymatic reaction. The increase in fluorescence intensity of sodium fluorescein was measured at lambda ex= 490 nm and lambda em= 514 nm. Under the optimum conditions the assay responded linearly to urea concentration in the range of 0.25 to 3 mM with a correlation coefficient of 0.996. This study also demonstrated the feasibility of a fluorometric method for urea determination with fiber optic detection. The probe response also displayed good linearity in the range of 0.10 to 1.75 mM urea.

引用

页码：1543 / 1551

页数：9

共 17 条

[1]

ABDELLATIF MS, 1988, ANAL LETT, V21, P943

[2] UREASE .4. ITS REACTION WITH N-ETHYLMALEIMIDE AND WITH SILVER ION [J].