Benzoic acid fermentation from starch and cellulose via a plant-like β-oxidation pathway in Streptomyces maritimus

被引:27
|
作者
Noda, Shuhei [1 ]
Kitazono, Eiichi [2 ]
Tanaka, Tsutomu [1 ]
Ogino, Chiaki [1 ]
Kondo, Akihiko [1 ]
机构
[1] Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] TEIJIN Holdings USA Inc, New York, CA 95035 USA
来源
MICROBIAL CELL FACTORIES | 2012年 / 11卷
关键词
Streptomyces; Benzoic acid; Endo-glucanase; Cellulose; PSEUDOMONAS-PUTIDA S12; ESCHERICHIA-COLI; BIOSYNTHESIS; GLUCOSE; GENE; PROTEINS; LIVIDANS; CLONING; PHENOL; STRAIN;
D O I
10.1186/1475-2859-11-49
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Benzoic acid is one of the most useful aromatic compounds. Despite its versatility and simple structure, benzoic acid production using microbes has not been reported previously. Streptomyces are aerobic, Gram-positive, mycelia-forming soil bacteria, and are known to produce various kinds of antibiotics composed of many aromatic residues. S. maritimus possess a complex amino acid modification pathway and can serve as a new platform microbe to produce aromatic building-block compounds. In this study, we carried out benzoate fermentation using S. maritimus. In order to enhance benzoate productivity using cellulose as the carbon source, we constructed endo-glucanase secreting S. maritimus. Results: After 4 days of cultivation using glucose, cellobiose, or starch as a carbon source, the maximal level of benzoate reached 257, 337, and 460 mg/l, respectively. S. maritimus expressed beta-glucosidase and high amylase-retaining activity compared to those of S. lividans and S. coelicolor. In addition, for effective benzoate production from cellulosic materials, we constructed endo-glucanase-secreting S. maritimus. This transformant efficiently degraded the phosphoric acid swollen cellulose (PASC) and then produced 125 mg/l benzoate. Conclusions: Wild-type S. maritimus produce benzoate via a plant-like beta-oxidation pathway and can assimilate various carbon sources for benzoate production. In order to encourage cellulose degradation and improve benzoate productivity from cellulose, we constructed endo-glucanase-secreting S. maritimus. Using this transformant, we also demonstrated the direct fermentation of benzoate from cellulose. To achieve further benzoate productivity, the L-phenylalanine availability needs to be improved in future.
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页数:10
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