Production of bioemulsifier by Bacillus subtilis, Alcaligenes faecalis and Enterobacter species in liquid culture

被引：50
作者：
Toledo, F. L. [1 ]
Gonzalez-Lopez, J. [1 ]
Calvo, C. [1 ]
机构：
[1] Univ Granada, Inst Water Res, Dept Microbiol, Grp Environm Microbiol, E-18071 Granada, Spain
来源：
BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 17期
关键词：
Bacillus subtilis; Alcaligenes faecalis; Enterobacter; bioemulsifiers; hydrocarbon;
D O I：
10.1016/j.biortech.2007.08.055
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Three bacterial strains isolated from waste crude oil were selected due to their capacity of growing in the presence of hydrocarbons and production of bioemulsifier. The genetic identification (PCR of the 16S rDNA gene using fD1 and rD1 primers) of these strains showed their affiliation to Bacillus subtilis, Alcaligenes faecalis and Enterobacter sp. These strains were able to emulsify n-octane, toluene, xylene, mineral oils and crude oil, look promising for bioremediation application. Finally, chemical composition, emulsifying activity and surfactant activity of the blopolymers produced by tile selected strains were studies under different culture conditions. Our results showed that chemical and functional properties of tile bioemulsifiers were affected by the carbon source added to tile growth media. (c) 2007 Elsevier Ltd. All rights reserved.
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页码：8470 / 8475
页数：6
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