Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules

被引：91

作者：

Lee, Somin Eunice ^{[1
,5
,6
]}

Chen, Qian ^{[2
,3
]}

Bhat, Ramray ^{[1
,7
]}

Petkiewicz, Shayne ^{[2
,3
]}

Smith, Jessica M. ^{[2
,3
]}

Ferry, Vivian E. ^{[2
,3
]}

Correia, Ana Luisa ^{[1
]}

Alivisatos, A. Paul ^{[2
,4
]}

Bissell, Mina J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Life Sci Div, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Mat Sci, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Chem & Mat Sci & Engn, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Kavli Energy NanoSci Inst, Berkeley, CA 94720 USA

[5] Univ Michigan, Dept Elect & Comp Engn, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48109 USA

[7] Indian Inst Sci, Dept Mol Reprod Dev & Genet, Bangalore 560012, Karnataka, India

来源：

NANO LETTERS | 2015年 / 15卷 / 07期

基金：

美国国家科学基金会;

关键词：

plasmon; single-molecule; secreted molecule; imaging; gold nanoparticle; MATRIX METALLOPROTEINASES; TISSUE ARCHITECTURE; MAMMARY-GLAND; NANOPARTICLE; GOLD; MORPHOGENESIS; CELLS; PROTEINASES; MEMBRANE; CANCER;

D O I：

10.1021/acs.nanolett.5b01161

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Plasmon rulers, consisting of pairs of gold nanoparticles, allow single-molecule analysis without photobleaching or blinking; however, current plasmon rulers are irreversible, restricting detection to only single events. Here, we present a reversible plasmon ruler, comprised of coupled gold nanoparticles linked by a single aptamer, capable of binding individual secreted molecules with high specificity. We show that the binding of target secreted molecules to the reversible plasmon ruler is characterized by single-molecule sensitivity, high specificity, and reversibility. Such reversible plasmon rulers should enable dynamic and adaptive live-cell measurement of secreted single molecules in their local microenvironment.

引用

页码：4564 / 4570

页数：7

共 31 条

[31] Aptamer induced multicoloured Au NCs-MoS2 "switch on" fluorescence resonance energy transfer biosensor for dual color simultaneous detection of multiple tumor markers by single wavelength excitation
Xu, Shenghao
Feng, Xiuying
Gao, Teng
Liu, Gufan
Mao, Yaning
Lin, Jiehua
Yu, Xijuan
Luo, Xiliang
ANALYTICA CHIMICA ACTA, 2017, 983 : 173 - 180

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