Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab

被引：16

作者：

Anisimova, V. E. ^{[1
]}

Rebrikov, D. V. ^{[1
]}

Zhulidov, P. A. ^{[1
]}

Staroverov, D. B. ^{[1
]}

Lukyanov, S. A. ^{[1
]}

Shcheglov, A. S. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2006年 / 71卷 / 05期

关键词：

duplex-specific nuclease (DSN); sugar nonspecific nuclease; renaturation; activation; specificity;

D O I：

10.1134/S0006297906050075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We overexpressed duplex-specific nuclease (DSN) from Kamchatka crab in Escherichia coli cells and developed procedures for purification, renaturation, and activation of this protein. We demonstrated identity of the properties of the native and recombinant DSN. We also successfully applied the recombinant DSN for full-length cDNA library normalization.

引用

页码：513 / 519

页数：7

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