Ciprofloxacin-induces free radical production in rat cerebral microsomes

被引：3

作者：

Gurbay, Aylin ^{[1
,2
,3
]}

Gonthier, Brigitte ^{[2
,4
]}

Daveloose, Denis ^{[3
]}

Barret, Luc ^{[2
]}

Favier, Alain ^{[5
]}

Hincal, Filiz ^{[1
]}

机构：

[1] Hacettepe Univ, Fac Pharm, Dept Pharmaceut Toxicol, Ankara, Turkey

[2] Univ Grenoble Alpes, Lab Med Legale & Toxicol, Grenoble, France

[3] Ctr Rech Serv Sante Armees, Serv Biophys, La Tronche, France

[4] Univ Grenoble Alpes, Hypoxie & Physiopathol Cardiovasc & Resp HP2, INSERM U1042, Grenoble, France

[5] CEA, Serv Chim Inorgan & Biol, Lab Les Acides Nucle, Dept Rech Fondamentale Matiere Condensee, Grenoble, France

来源：

FREE RADICAL RESEARCH | 2019年 / 53卷 / 04期

关键词：

Cerebral microsomes; ciprofloxacin; electron spin resonance; free radicals; protector agents; CEREBROSPINAL-FLUID; GABA(A) RECEPTOR; NADPH OXIDATION; LIPID RADICALS; FLUOROQUINOLONES; CELLS; ZINC; PENETRATION; METABOLISM; QUINOLONES;

D O I：

10.1080/10715762.2019.1591622

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the presence of ciprofloxacin (CPFX), free radical adduct formation was demonstrated in rat cerebral microsomes using a spin trap alpha-(4-pyridyl-1-oxide)-N-tert-butyl-nitrone by electron spin resonance spectroscopy. Active microsomes, dihydronicotinamide-adenine dinucleotide phosphate, and ciprofloxacin were necessary for the formation of a spin trap/radical adduct. Adduct formation increased dose-dependently at 0.5-1 mM CPFX concentration for 180 min, and 0.3-1 mM concentration level for 240 min. The addition of SKF 525A, ZnCl2 or desferrioxamine to the incubation system caused complete inhibition of the radical formation. However, pretreatment of microsomal system with superoxide dismutase (SOD) did not induce any protective effect. Induction of lipid peroxidation, and depletion of thiol levels by CPFX were also shown in the system. These results strongly suggested that CPFX produces free radical(s) in the cerebral microsomes of rats.

引用

页码：397 / 404

页数：8

共 51 条

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