Molecular cloning, functional expression and characterization of d-limonene synthase from Agastache rugosa

被引：23

作者：

Maruyama, T ^{[1
]}

Saeki, D ^{[1
]}

Ito, M ^{[1
]}

Honda, G ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Pharmacognosy, Kyoto 6068501, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2002年 / 25卷 / 05期

关键词：

d-limonene synthase; monoterpene biosynthesis; Agastache rugosa (Labiatae); cDNA cloning;

D O I：

10.1248/bpb.25.661

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We cloned the gene of d-limonene synthase (ArLMS) from Agastache rugosa (Labiatae). The function of ArLMS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. ArLMS consisted of 2077 nucleotides including 1839 bp of coding sequence that encodes a protein of 613 amino acids. This protein has a 60kDa molecular weight, which is identical to that of d-limonene synthase from Schizonepeta tenuifolia (Labiatae). The deduced amino acid sequence of ArLMS shows high homology with the known d- and I-limonene synthases from Labiatae plants. Here, we discussed the amino acid residues responsible for the stereochemical regulation in limonene biosynthesis.

引用

页码：661 / 665

页数：5

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