Roselipins, inhibitors of diacylglycerol acyltransferase, produced by Gliocladium roseum KF-1040

被引：52

作者：

Tomoda, H

Ohyama, Y

Abe, T

Tabata, N

Namikoshi, M

Yamaguchi, Y

Masuma, R

Ömura, S ^{[1
]}

机构：

[1] Kitasato Univ, Kitasato Inst, Biol Funct Res Ctr, Minato Ku, Tokyo 1088642, Japan

[2] Kitasato Univ, Grad Sch Pharmaceut Sci, Minato Ku, Tokyo 1088642, Japan

[3] Tokyo Univ Fisheries, Minato Ku, Tokyo 1088477, Japan

来源：

JOURNAL OF ANTIBIOTICS | 1999年 / 52卷 / 08期

关键词：

D O I：

10.7164/antibiotics.52.689

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gliocladium roseum KF-1040, a marine isolate, was found to produce a series of new inhibitors of diacylglycerol acyltransferase (DGAT). Four active compounds, designated roselipins 1A, 1B, 2A and 2B, were isolated from the fermentation broth of the producing strain by solvent extraction, ODS column chromatography and preparative HPLC. The highest production of roselipins tvas observed when cultured in the medium containing natural sea water. Roselipins inhibit DGAT activity with IC50 values of 15 similar to 22 mu M a in an enzyme assay system using rat liver microsomes.

引用

页码：689 / 694

页数：6

共 9 条

[1]

Bell RM, 1983, ENZYMES, V16, P87

[2]

DOMSCH KH, 1980, COMPENDIUM SOIL FUNG, P371

[3]

MAYOREK N, 1985, J BIOL CHEM, V260, P6528

[4] Roselipins, novel fungal metabolites having a highly methylated fatty acid modified with a mannose and an arabinitol [J].