Treatment of PAHs in contaminated soil by extraction with aqueous DNA followed by biodegradation with a pure culture of Sphingomonas sp.

被引：21

作者：

Navarro, Ronald R. ^{[1
]}

Iimura, Yosuke ^{[1
]}

Ichikawa, Hiroyasu ^{[1
]}

Tatsumi, Kenji ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058569, Japan

来源：

CHEMOSPHERE | 2008年 / 73卷 / 09期

关键词：

Carcinogenic; PAH solubilization; Salmon DNA; Soil remediation; Surfactant;

D O I：

10.1016/j.chemosphere.2008.08.004

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in aqueous deoxyribonucleic acid (DNA) solution from contaminated soil washing was investigated. Initial data with a model effluent consisting of anthracene, phenanthrene, pyrene and benzolalpyrene that were individually dissolved in 1% aqueous DNA solution confirmed their positive degradation by Sphingomonas sp. at around 10(8)CFUmL(-1) initial cell loading. For anthracene and phenanthrene, complete removal was achieved within 1 h treatment. Degradation of pyrene and benzo[a]pyrene took a relatively longer time of a few days and weeks, respectively. DNA-dissolved PAHs were also degraded relatively faster than PAH crystals in aqueous medium to suggest that the binding of the PAHs in the polymer does not pose serious constraint to bacterial uptake. The DNA was stable against the PAH-degrading bacteria. Parallel experiments with actual DNA solutions obtained during pyrene extraction from an artificially spiked soil also showed similar results. Close to 100% pyrene degradation was achieved after 1 d treatment. With its chemical stability, the cell-treated DNA was re-used up to four cycles without a considerable decline in extraction performance. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1414 / 1419

页数：6

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