Colorectal cancer is a common malignancy and one of the major causes of cancer-related deaths worldwide. Similar to other human cancers, tumor metastasis is the biggest obstacle in the clinical treatment of colorectal cancer. In this study, we explored the functional role of SLIT2 in colon tumor metastasis and the relevant molecular mechanisms. Immunohistochemistry, Western blotting, and quantitative reverse transcription-polymerase chain reaction were used to measure SLIT2 expression in colorectal tumor tissues in the presence or absence of metastasis. Wound-healing assays, Transwell assays, Western blotting, and immunofluorescence assays were used to examine the effects of SLIT2 on SW480 and NCM460 cell migration and the epithelial-to-mesenchymal transition (EMT). An AKT inhibitor was introduced to examine the mechanism underlying SLIT2-mediated suppression of NCM460 cell migration. Higher SLIT2 expression was detected in metastasis-positive tumor tissues, and this upregulation was beneficial for the overall survival of patients with colorectal cancer. Either the addition of purified SLIT2 or overexpression of SLIT2 inhibited SW480 cell migration, whereas the depletion of SLIT2 with shRNA enhanced the migratory ability of NCM460 cells. Meanwhile, SLIT2 depletion also induced beta-catenin accumulation and altered the expression levels of several molecules related to EMT in NCM460 cells. AKT inhibition abrogated the effects of SLIT2 depletion on EMT and migration in NCM460 cells. SLIT2 suppresses colon tumor metastasis, and it exerts its suppressive activity against colorectal cancer metastasis by restraining AKT signaling and EMT, thus making it a potential clinical prognosis marker in colorectal cancer.
机构:
Shenzhen Tradit Chinese Med Hosp, Dept Gen Surg, Shenzhen, Peoples R ChinaShenzhen Tradit Chinese Med Hosp, Dept Gen Surg, Shenzhen, Peoples R China
Li, Jia
Jiang, Ji Ling
论文数: 0引用数: 0
h-index: 0
机构:
Shenzhen Tradit Chinese Med Hosp, Dept Gen Surg, Shenzhen, Peoples R ChinaShenzhen Tradit Chinese Med Hosp, Dept Gen Surg, Shenzhen, Peoples R China
Jiang, Ji Ling
Chen, Yi Mei
论文数: 0引用数: 0
h-index: 0
机构:
Shenzhen Women & Childrens Hlth Care Hosp, Dept Breast Surg, Shenzhen, Peoples R ChinaShenzhen Tradit Chinese Med Hosp, Dept Gen Surg, Shenzhen, Peoples R China
Chen, Yi Mei
Lu, Wei Qi
论文数: 0引用数: 0
h-index: 0
机构:
Guangzhou Univ Chinese Med, Dept Gastrointestinal Surg, Affiliated Hosp 1, 16 Airport Rd, Guangzhou 510405, Peoples R ChinaShenzhen Tradit Chinese Med Hosp, Dept Gen Surg, Shenzhen, Peoples R China
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
Borzacchiello, Luigi
Tranchese, Roberta Veglia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
Tranchese, Roberta Veglia
Grillo, Roberta
论文数: 0引用数: 0
h-index: 0
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
Grillo, Roberta
Arpino, Roberta
论文数: 0引用数: 0
h-index: 0
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
Arpino, Roberta
Mosca, Laura
论文数: 0引用数: 0
h-index: 0
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
Mosca, Laura
Cacciapuoti, Giovanna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
Cacciapuoti, Giovanna
Porcelli, Marina
论文数: 0引用数: 0
h-index: 0
机构:
Univ Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, ItalyUniv Campania Luigi Vanvitelli, Dept Precis Med, Via Luigi De Crecchio 7, I-80138 Naples, Italy
机构:
Fudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R ChinaFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Pan, Han
Song, Yimeng
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R ChinaFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Song, Yimeng
Zhang, Hang
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R ChinaFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Zhang, Hang
Bai, Yang
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R ChinaFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Bai, Yang
Konishi, Teruaki
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Quantum & Radiol Sci & Technol, Inst Quantum Life Sci, Inage, Chiba 2638555, Japan
Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Inage, Chiba 2638555, JapanFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Konishi, Teruaki
Kobayashi, Alisa
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Quantum & Radiol Sci & Technol, Inst Quantum Life Sci, Inage, Chiba 2638555, Japan
Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Inage, Chiba 2638555, JapanFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Kobayashi, Alisa
Shao, Chunlin
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R ChinaFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Shao, Chunlin
Pan, Yan
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R ChinaFudan Univ, Shanghai Med Coll, Inst Radiat Med, 2094 Xie Tu Rd, Shanghai 200032, Peoples R China
Pan, Yan
AMERICAN JOURNAL OF CANCER RESEARCH,
2021,
11
(01):
: 61
-
U29
机构:
Zaozhuang Municipal Hosp, Dept Pediat Surg, Zaozhuang 277102, Shandong, Peoples R ChinaZaozhuang Municipal Hosp, Dept Pediat Surg, Zaozhuang 277102, Shandong, Peoples R China
Qian, Cheng
Liu, Qiang
论文数: 0引用数: 0
h-index: 0
机构:
Second Peoples Hosp Nantong, Dept Urinary Surg, 298 Xinhua Rd, Nantong 226002, Jiangsu, Peoples R ChinaZaozhuang Municipal Hosp, Dept Pediat Surg, Zaozhuang 277102, Shandong, Peoples R China