Axenic culture of fastidious and intracellular bacteria

被引：35

作者：

Singh, Sudhir ^{[1
]}

Eldin, Carole ^{[1
]}

Kowalczewska, Malgorzata ^{[1
]}

Raoult, Didier ^{[1
]}

机构：

[1] Fac Med Marseille, Unite Mixte Rech 7278, INSERM, CNRS,URMITE,Inst Rech Dev 198,U1095, F-13385 Marseille 5, France

来源：

TRENDS IN MICROBIOLOGY | 2013年 / 21卷 / 02期

关键词：

axenic medium; fastidious bacteria; intracellular bacteria; complex microbiota; growth; culture; FINISHED GENOME SEQUENCE; LEGIONELLA-PNEUMOPHILA; GUT MICROBIOTA; RICKETTSIA-PROWAZEKII; TROPHERYMA-WHIPPLEI; COXIELLA-BURNETII; BLOOD AGAR; CULTIVATION; DIAGNOSIS; STRAINS;

D O I：

10.1016/j.tim.2012.10.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The culture of microorganisms has been the basis of microbiology. However, revolutionary tools such as metagenomics have made it possible to describe uncultivated bacteria, but several breakthroughs have occurred in culture leading to a revival of these techniques. In this review we focus on new applications that have successfully cultivated previously uncultivated bacteria. We also review the axenic cultivation of intracellular bacteria such as Tropheryma whipplei and Coxiella burnetii. These successes provide new tools for the design of axenic media for intracellular bacteria, such as Rickettsiae and Chlamydiae, or historically uncultivable pathogens, such as Mycobacterium leprae and Treponema pallidum. The future axenic culture of these microorganisms will facilitate antibiotic susceptibility testing and will provide insight into their microbial ecology and pathogenicity.

引用

页码：92 / 99

页数：8

共 71 条

[1] Diagnosis of Lyme borreliosis [J].