Optimization of Expression Conditions Enhances Production of Sepiapterin, a Precursor for Tetrahydrobiopterin Biosynthesis, in Recombinant Escherichia coli

被引：0

作者：

Park, Eun-Hee ^{[1
]}

Lee, Won-Heong ^{[2
]}

Jang, Mi-Hee ^{[1
]}

Kim, Myoung-Dong ^{[1
]}

机构：

[1] Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon 200701, South Korea

[2] Chonnam Natl Univ, Dept Bioenergy Sci & Technol, Kwangju 500757, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 25卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

Sepiapterin; GTP cyclohydrolase 1 (GCH1); 6-pyruvoyltetrahydropterin synthase (PTPS); Escherichia coli; DEPRESSION; BIOPTERIN; REDUCTASE; COFACTOR; INJURY;

D O I：

10.4014/jmb.1506.06034

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sepiapterin is a precursor for the synthesis of tetrahydrobiopterin (BH4), which is a well-known cofactor for aromatic amino acid hydroxylation and nitric oxide synthesis in higher mammals. In this study, a recombinant Escherichia coli BL21(DE3) strain harboring cyanobacterial guanosine 5'-triphosphate cyclohydrolase 1 (GCH1) and human 6-pyruvoyltetrahydropterin synthase (PTPS) genes was constructed to produce sepiapterin. The optimum conditions for T7 promoter-driven expression of GCH1 and PTPS were 30 degrees C and 0.1 mM isopropyl-beta-D-thioglucopyranoside (IPTG). The maximum sepiapterin concentration of 88.1 +/- 2.4 mg/l was obtained in a batch cultivation of the recombinant E. coli, corresponding to an 18-fold increase in sepiapterin production compared with the control condition (37 degrees C and 1 mM IPTG).

引用

页码：1709 / 1713

页数：5

共 21 条

[1] TETRAHYDROBIOPTERIN METABOLISM IN THE TEMPORAL-LOBE OF PATIENTS DYING WITH SENILE DEMENTIA OF ALZHEIMER TYPE
BARFORD, PA
BLAIR, JA
EGGAR, C
HAMON, C
MORAR, C
WHITBURN, SB
[J]. JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 1984, 47 (07) : 736 - 738
[2] Sepiapterin inhibits cell proliferation and migration of ovarian cancer cells via down-regulation of p70S6K-dependent VEGFR-2 expression
Cho, Young-Rak
Kim, Soo Hyeon
Ko, Hee Young
Kim, Myoung-Dong
Choi, Shin Wook
Seo, Dong-Wan
[J]. ONCOLOGY REPORTS, 2011, 26 (04) : 861 - 867
[3] TETRAHYDROBIOPTERIN - EFFICACY IN ENDOGENOUS-DEPRESSION AND PARKINSONS-DISEASE
CURTIUS, HC
MULDNER, H
NIEDERWIESER, A
[J]. JOURNAL OF NEURAL TRANSMISSION, 1982, 55 (04) : 301 - 308
[4] Tetrahydrobiopterin as a Novel Therapeutic Intervention for Autism
Frye, Richard E.
Huffman, Lynne C.
Elliott, Glen R.
[J]. NEUROTHERAPEUTICS, 2010, 7 (03) : 241 - 249
[5] KAUFMAN S, 1958, J BIOL CHEM, V230, P931
[6] KWON NS, 1989, J BIOL CHEM, V264, P20496
[7] Effects of Sepiapterin Infusion on Renal Oxygenation and Early Acute Renal Injury After Suprarenal Aortic Clamping in Rats
Legrand, Matthieu
Kandil, Asli
Payen, Didier
Ince, Can
[J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2011, 58 (02) : 192 - 198
[8] TRYPTOPHAN HYDROXYLATION - MEASUREMENT IN PINEAL GLAND BRAINSTEM AND CARCINOID TUMOR
LOVENBERG, W
JEQUIER, E
SJOERDSMA, A
[J]. SCIENCE, 1967, 155 (3759) : 217 - +
[9] MILLER H, 1987, METHOD ENZYMOL, V152, P145
[10] IMMUNOLOGICAL STUDIES ON THE PARTICIPATION OF 6-PYRUVOYL TETRAHYDROPTERIN (2'-OXO) REDUCTASE, AN ALDOSE REDUCTASE, IN TETRAHYDROBIOPTERIN BIOSYNTHESIS
MILSTIEN, S
KAUFMAN, S
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (02) : 845 - 850

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