Antibacterial effect of microvesicles released from human neutrophilic granulocytes

被引：177

作者：

Timar, Csaba I. ^{[1
]}

Lorincz, Akos M. ^{[1
]}

Csepanyi-Koemi, Roland ^{[1
]}

Valyi-Nagy, Anna ^{[1
]}

Nagy, Gyoergy ^{[2
]}

Buzas, Edit I. ^{[2
]}

Ivanyi, Zsolt ^{[3
]}

Kittel, Agnes ^{[4
]}

Powell, David W. ^{[5
,6
]}

McLeish, Kenneth R. ^{[5
,6
,7
]}

Ligeti, Erzsebet ^{[1
]}

机构：

[1] Semmelweis Univ, Dept Physiol, H-1094 Budapest, Hungary

[2] Semmelweis Univ, Dept Genet Cell Biol & Immunol, H-1094 Budapest, Hungary

[3] Semmelweis Univ, Clin Anaesthesiol & Intens Care, H-1094 Budapest, Hungary

[4] Hungarian Acad Sci, Inst Expt Med, Budapest, Hungary

[5] Univ Louisville, Dept Med, Louisville, KY 40292 USA

[6] Univ Louisville, Dept Biochem & Mol Biol, Louisville, KY 40292 USA

[7] Robley Rex VA Med Ctr, Louisville, KY USA

来源：

BLOOD | 2013年 / 121卷 / 03期

关键词：

MEMBRANE-VESICLES; MICROPARTICLES; STIMULATE; MECHANISM; PROTEINS; EXOSOMES;

D O I：

10.1182/blood-2012-05-431114

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Cell-derived vesicles represent a recently discovered mechanism for intercellular communication. We investigated their potential role in interaction of microbes with host organisms. We provide evidence that different stimuli induced isolated neutrophilic granulocytes to release microvesicles with different biologic properties. Only opsonized particles initiated the formation of microvesicles that were able to impair bacterial growth. The antibacterial effect of neutrophil-derived microvesicles was independent of production of toxic oxygen metabolites and opsonization or engulfment of the microbes, but depended on beta(2) integrin function, continuous actin remodeling, and on the glucose supply. Neutrophil-derived microvesicles were detected in the serum of healthy donors, and their number was significantly increased in the serum of bacteremic patients. We propose a new extracellular mechanism to restrict bacterial growth and dissemination. (Blood. 2013;121(3):510-518)

引用

页码：510 / 518

页数：9

共 41 条

[1] Intercellular transfer of the oncogenic receptor EGFrvIII by microvesicles derived from tumour cells [J].

Al-Nedawi, Khalid ;

Meehan, Brian ;

Micallef, Johann ;

Lhotak, Vladimir ;

May, Linda ;

Guha, Abhijit ;

Rak, Janusz .

NATURE CELL BIOLOGY, 2008, 10 (05) :619-U24

[2] Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo [J].

Bhatnagar, Sanchita ;

Shinagawa, Kazuhiko ;

Castellino, Francis J. ;

Schorey, Jeff Rey S. .

BLOOD, 2007, 110 (09) :3234-3244

[3] Restoration of NET formation by gene therapy in CGD controls aspergillosis [J].

Bianchi, Matteo ;

Hakkim, Abdul ;

Brinkmann, Volker ;

Siler, Ulrich ;

Seger, Reinhard A. ;

Zychlinsky, Arturo ;

Reichenbach, Janine .

BLOOD, 2009, 114 (13) :2619-2622

[4] Neutrophil extracellular traps kill bacteria [J].

Brinkmann, V ;

Reichard, U ;

Goosmann, C ;

Fauler, B ;

Uhlemann, Y ;

Weiss, DS ;

Weinrauch, Y ;

Zychlinsky, A .

SCIENCE, 2004, 303 (5663) :1532-1535

[5] Exosomes/microvesicles as a mechanism of cell-to-cell communication [J].

Camussi, Giovanni ;

Deregibus, Maria C. ;

Bruno, Stefania ;

Cantaluppi, Vincenzo ;

Biancone, Luigi .

KIDNEY INTERNATIONAL, 2010, 78 (09) :838-848

[6] Shedding microvesicles: artefacts no more [J].

Cocucci, Emanuele ;

Racchetti, Gabriella ;

Meldolesi, Jacopo .

TRENDS IN CELL BIOLOGY, 2009, 19 (02) :43-51

[7] Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-β signaling [J].

Cummins, Timothy D. ;

Barati, Michelle T. ;

Coventry, Susan C. ;

Salyer, Sarah A. ;

Klein, Jon B. ;

Powell, David W. .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2010, 1804 (04) :653-661

[8] Proteomic and functional characterisation of platelet microparticle size classes [J].

Dean, William L. ;

Lee, Menq J. ;

Cummins, Timothy D. ;

Schultz, David J. ;

Powell, David W. .

THROMBOSIS AND HAEMOSTASIS, 2009, 102 (04) :711-718

[9] Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation [J].

Duarte, Tonya Azevedo ;

Noronha-Dutra, Alberto Augusto ;

Nery, Joilda Silva ;

Ribeiro, Samantha Brum ;

Pitanga, Thassila Nogueira ;

Lapa e Silva, Jose R. ;

Arruda, Sergio ;

Boechat, Neio .

TUBERCULOSIS, 2012, 92 (03) :218-225

[10] Polymorphonuclear neutrophil-derived ectosomes interfere with the maturation of monocyte-derived dendritic cells [J].

Eken, Ceylan ;

Gasser, Olivier ;

Zenhaeusern, Gabriela ;

Oehri, Ineke ;

Hess, Christoph ;

Schifferli, Juerg A. .

JOURNAL OF IMMUNOLOGY, 2008, 180 (02) :817-824

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