The impact of TGF-β1 on the mRNA expression of TβR I, TβR II, Smad4 and the invasiveness of the JEG-3 placental choriocarcinoma cell line

被引：14
作者：
Li, Yuhong [1 ]
Xu, Qian [1 ]
Zhang, Zhuo [1 ]
Liu, Shaochen [1 ]
Shi, Changhua [1 ]
Tan, Yusi [1 ]
机构：
[1] Chengde Med Coll, Dept Basic Med, Chengde 067000, Hebei, Peoples R China
来源：
ONCOLOGY LETTERS | 2012年 / 4卷 / 06期
关键词：
transforming growth factor-beta 1; transforming growth factor-beta 1 receptor type I; transforming growth factor-beta 1 receptor type II; Smad4; matrix metalloprotease-9; tissue inhibitors; of metalloprotease-1; choriocarcinoma; GROWTH-FACTOR-BETA; CANCER; INVASION; METASTASIS; ROLES;
D O I：
10.3892/ol.2012.906
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Human choriocarcinoma is one of the most aggressive malignant tumors characterized by early hematogenous spread to lung and brain tissues, and may be a cause of death in patients. Choriocarcinoma may occur following pregnancy and during implantation; however, trophoblastic invasion in human pregnancy is tightly regulated. The transforming growth factor-beta 1 (TGF-beta 1) has been suggested to play a role in controlling this process. In this study, we investigated the impact of TGF-beta 1 on invasion, as well as its sites of action in the TGF-beta 1/Smad pathway using a JEG-3 choriocarcinoma cell line. Following the treatment of cells with different doses of TGF-beta 1, cell invasion was observed. We also detected the expression of TGF-beta receptor type I (T beta R I) and TGF-beta receptor type II (T beta R II), Smad4, matrix metalloprotease (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 in JEG-3 cells. Our data demonstrated that TGF-beta 1 promoted the invasive capability of JEG-3 cells depending on the downregulation of T beta R I, T beta R II, Smad4 and the upregulation of MMP-9 and TIMP-I. These observations suggest that TGF-beta 1 may play a critical role in the initiation of the trophoblastic invasion process.
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页码：1344 / 1348
页数：5
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