SEA-4 is a Marker of Human Deciduous Periodontal Ligament Stem Cells

被引：21

作者：

Fukushima, H. ^{[2
]}

Kawanabe, N. ^{[1
]}

Murata, S. ^{[2
]}

Ishihara, Y. ^{[2
]}

Yanagita, T. ^{[1
]}

Balam, T. A. ^{[2
]}

Yamashiro, T. ^{[2
]}

机构：

[1] Okayama Univ Hosp, Dept Orthodont, Okayama 7008525, Japan

[2] Okayama Univ, Dept Orthodont, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008530, Japan

来源：

JOURNAL OF DENTAL RESEARCH | 2012年 / 91卷 / 10期

基金：

日本学术振兴会;

关键词：

human deciduous periodontal ligament; stage-specific embryonic antigen-4; adult stem cells; clonal assays; multipotential differentiation; flow cytometry; BONE-MARROW-TRANSPLANTATION; HIGH-DOSE CHEMOTHERAPY; OSTEOGENESIS IMPERFECTA; MESENCHYMAL CELLS; BREAST-CANCER; STROMAL CELLS; EPITHELIUM; CHILDREN; THERAPY; IDENTIFICATION;

D O I：

10.1177/0022034512458123

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Although human deciduous teeth are an ideal source of adult stem cells, no method for identifying deciduous periodontal ligament (D-PDL) stem cells has so far been developed. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 is a marker that could be used to isolate D-PDL stem cells. The isolated D-PDL cells met the minimum criteria for mesenchymal stem cells (MSCs): They showed plastic adherence, specific-surface antigen expression, and multipotent differentiation potential. SSEA-4+ D-PDL cells were detected in vitro and in vivo. A flow cytometric analysis demonstrated that 22.7% of the D-PDL cells were positive for SSEA-4. SSEA-4+ clonal D-PDL cells displayed multilineage differentiation potential: They were able to differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. A clonal assay demonstrated that 61.5% of the SSEA-4+ D-PDL cells had adipogenic, osteogenic, and chondrogenic potential. Our present study demonstrated that SSEA-4+ D-PDL cells are a subset of multipotent stem cells. Hence, SSEA-4 is a specific marker that can be used to identify D-PDL stem cells.

引用

页码：955 / 960

页数：6

共 30 条

[1] ISOLATION OF A CANDIDATE HUMAN HEMATOPOIETIC STEM-CELL POPULATION [J].

BAUM, CM ;

WEISSMAN, IL ;

TSUKAMOTO, AS ;

BUCKLE, AM ;

PEAULT, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :2804-2808

[2] Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells [J].

Colter, DC ;

Sekiya, I ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (14) :7841-7845

[3] Establishment of a Mesenchymal Stem Cell Bank [J].

Cooper, Khushnuma ;

Viswanathan, Chandra .

STEM CELLS INTERNATIONAL, 2011, 2011

[4] HUMAN MANDIBLE BONE DEFECT REPAIR BY THE GRAFTING OF DENTAL PULP STEM/PROGENITOR CELLS AND COLLAGEN SPONGE BIOCOMPLEXES [J].

d'Aquino, Riccardo ;

De Rosa, Alfredo ;

Lanza, Vladimiro ;

Tirino, Virginia ;

Laino, Luigi ;

Graziano, Antonio ;

Desiderio, Vincenzo ;

Laino, Gregorio ;

Papaccio, Gianpaolo .

EUROPEAN CELLS & MATERIALS, 2009, 18 :75-83

[5] Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

[6] PERMANENT COVERAGE OF LARGE BURN WOUNDS WITH AUTOLOGOUS CULTURED HUMAN EPITHELIUM [J].

GALLICO, GG ;

OCONNOR, NE ;

COMPTON, CC ;

KEHINDE, O ;

GREEN, H .

NEW ENGLAND JOURNAL OF MEDICINE, 1984, 311 (07) :448-451

[7] SSEA-4 identifies mesenchymal stem cells from bone marrow [J].

Gang, Eun J. ;

Bosnakovski, Darko ;

Figueiredo, Camila A. ;

Visser, Jan W. ;

Perlingeiro, Rita C. R. .

BLOOD, 2007, 109 (04) :1743-1751

[8] Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].

Gronthos, S ;

Mankani, M ;

Brahim, J ;

Robey, PG ;

Shi, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630

[9] Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta [J].

Horwitz, EM ;

Prockop, DJ ;

Fitzpatrick, LA ;

Koo, WWK ;

Gordon, PL ;

Neel, M ;

Sussman, M ;

Orchard, P ;

Marx, JC ;

Pyeritz, RE ;

Brenner, MK .

NATURE MEDICINE, 1999, 5 (03) :309-313

[10] Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone [J].

Horwitz, EM ;

Gordon, PL ;

Koo, WKK ;

Marx, JC ;

Neel, MD ;

McNall, RY ;

Muul, L ;

Hofmann, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (13) :8932-8937

← 1 2 3 →