A one-stage cultivation process for the production of poly-3-(hydroxybutyrate-co-hydroxyvalerate) from olive mill wastewater by Haloferax mediterranei

被引：126
作者：
Alsafadi, Diya [1 ]
Al-Mashaqbeh, Othman [1 ]
机构：
[1] Royal Sci Soc, Ctr Sci Res, Amman 11941, Jordan
来源：
NEW BIOTECHNOLOGY | 2017年 / 34卷
关键词：
Polyhydroxyalkanoates; Olive mill wastewater; Haloferax mediterranei; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Haloarchaea; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE); POLYHYDROXYALKANOATES; CULTURE; WASTEWATERS; EFFLUENTS; STRAIN; STARCH; CARBON; ADH2; SP;
D O I：
10.1016/j.nbt.2016.05.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Olive mill wastewater (0MW), a highly polluting waste from the olive oil industry, was utilized as sole carbon source for the production of polyhydroxyalkanoate (PHA) by extremely halophilic Haloferax Mediterranei (H. mediterranei) in a one stage cultivation step. H. mediterranei showed remarkable cell growth and tolerated the inhibitory effect of polyphenols present in medium containing 25% of OMW. H. mediterranei cultivation conditions were optimized in medium containing 15% OMW by investigating several parameters that affect the production of PHA. The highest polymer yield (0.2 g/L) and PHA content (43% PHA/cell dry mass) were achieved at 37 degrees C, 170 rpm and 22% salt concentration. Analysis of the produced PHA revealed the production of copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) containing 6.5 mol% 3-hydroxyvalerate (3HV). The production of PHBHV was observed without the need for fermentation step or adding external carbon source. The PHBHV displayed reduced melting points at 140.1 degrees C and 154.4 degrees C when compared to homopolymer polyhydroxybutyrate. (C) 2016 Elsevier B.V. All rights reserved.
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页码：47 / 53
页数：7
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