Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus

被引：75

作者：

Chen, Yi-Pei ^{[1
,2
]}

Tseng, Ching-Ping ^{[2
]}

Liaw, Li-Ling ^{[1
]}

Wang, Chun-Lin ^{[1
]}

Chen, I-Ching ^{[1
]}

Wu, Wen-Jung ^{[1
]}

Wu, Ming-Der ^{[1
]}

Yuan, Gwo-Fang ^{[1
]}

机构：

[1] Food Ind Res & Dev Inst, Bioresource Collect & Res Ctr, Hsinchu, Taiwan

[2] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 14期

关键词：

monacolin K; polyketide synthases; Monascus pilosus; bacterial artificial chromosome;

D O I：

10.1021/jf800595k

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus, and it has the same structure as lovastatin, which is mainly produced by Aspergillus terreus. In the present study, a bacterial artificial chromosome (BAC) clone, mps01, was screened from the BAC library constructed from Monascus pilosus BCRC38072 genomic DNA. The putative monacolin K biosynthetic gene cluster was found within a 42 kb region in the mps01 clone. The deduced amino acid sequences encoded by the nine genes designated as mokA-mokl, which share over 54% similarity with the lovastatin biosynthetic gene cluster in A. terreus, were assumed to be involved in monacolin K biosynthesis. A gene disruption construct designed to replace the central part of mokA, a polyketide synthase gene, in wild-type M. pilosus BCRC38072 with a hygromycin B resistance gene through homologous recombination, resulted in a mokA-disrupted strain. The disruptant did not produce monacolin K, indicating that mokA encoded the PKS responsible for monacolin K biosynthesis in M. pilosus BCRC38072.

引用

页码：5639 / 5646

页数：8

共 24 条

[21] Polyketide synthase gene responsible for citrinin biosynthesis in Monascus purpureus [J].

Shimizu, T ;

Kinoshita, H ;

Ishihara, S ;

Sakai, K ;

Nagai, S ;

Nihira, T .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (07) :3453-3457

[22] Transformations of cyclic nonaketides by Aspergillus terreus mutants blocked for lovastatin biosynthesis at the lovA and lovC genes [J].

Sorensen, JL ;

Auclair, K ;

Kennedy, J ;

Hutchinson, CR ;

Vederas, JC .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (01) :50-59

[23] Monacolin N, a compound resulting from derailment of type I iterative polyketide synthase function en route to lovastatin [J].

Sorensen, JL ;

Vederas, JC .

CHEMICAL COMMUNICATIONS, 2003, (13) :1492-1493

[24] Lovastatin and beyond: The history of the HMG-CoA reductase inhibitors [J].

Tobert, JA .

NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (07) :517-526

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